Lamin B Antikörper

Antigen: Lamin B

Synonyme: 2459, 74/76, CG6944, D5, Dm, Dm(0), Dm0, Dm1, Dm2, DmelCG6944, DmLamin, Dmo, DmO, Dm[[0]], Dm[[1]], Dm[[2]], Dm[[mit]], Dm[[o]], DM[[O]], jf27, l(2)04643, l(2)25Ec, l(2)gdh-7, l(2)gdh7, l(2)jf27, lam, Lam Dm[[0]], Lam(Dm0), lamDm0, LamDm[[0], lamDm[[0]], LamDm[[0]], lamin, lamin Dm0, lamin Dm[[0]], Lamin Dm[[0]], Lam[[Dm0]], misg, nlam

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Western Blot (WB), Immunzytochemie (ICC), Immunhistochemie (Paraffinschnitte) (IHC (p)), Immunhistochemie (Gefrierschnitte) (IHC (fro))

Produktnummer: ABIN343459

Produktbeschreibung

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of human Lamin B,different from the related rat and mouse sequence by three amino acids.

Format: Affinity-purified

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Lamins are the major components of the nuclear lamina which underlies the nuclear envelope of eukaryotic cells. lamin B is a structural component of the long-sought-after spindle matrix that promotes microtubule assembly and organization in mitosis. Inspection of the deduced amino acid sequence of lamin B revealed the presence in coil 1B of the alpha-helical domain of a leucine heptad repeat region. Lamin B assembled into a matrix-like network in mitosis through a process that depended on the presence of the guanosine triphosphate-bound form of the small guanosine triphosphatase Ran.

Spezifität: Human, mouse, rat. No cross reactivity with other proteins.

Synonyme: 2459, 74/76, CG6944, D5, Dm, Dm(0), Dm0, Dm1, Dm2, DmelCG6944, DmLamin, Dmo, DmO, Dm[[0]], Dm[[1]], Dm[[2]], Dm[[mit]], Dm[[o]], DM[[O]], jf27, l(2)04643, l(2)25Ec, l(2)gdh-7, l(2)gdh7, l(2)jf27, lam, Lam Dm[[0]], Lam(Dm0), lamDm0, LamDm[[0], lamDm[[0]], LamDm[[0]], lamin, lamin Dm0, lamin Dm[[0]], Lamin Dm[[0]], Lam[[Dm0]], misg, nlam

Anwendungen

Applikationshinweise: Western blotting: At 1-2u/ml with the appropriate system to detect Lamin B in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 0.5-1u/ml to detect Lamin B in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Immunohistochemistry(F) At 0.5-1u/ml to detect Lamin B in formalin or acetone fixed tissues. Immunocytochemistry At 0.5-1u/ml to detect Lamin B in acetone fixed cell. Antigen retrieval by Pepsin and Trypsin is required. Other applications have not been tested. Optimal dilutions should be determined by end user.

Reinheit: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Forschungsgebiet: Zytoskelett

Beschränkungen: Nur für Forschungszwecke einsetzbar