WD Repeat Domain 83 (wdr83) Antikörper

Antigen: WD Repeat Domain 83 (wdr83)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Western Blot (WB), Immunzytochemie (ICC), Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN343454

Produktbeschreibung

Weitere Bezeichnung: Mitogen-activated protein organizer 1, MORG1

Immunogen: A synthetic peptide corresponding to a sequence near the N-terminal of human MORG1,identical to the related mouse and rat sequence.

Format: Affinity-purified

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: MORG1 (mitogen-activated protein kinase organizer 1), a member of the WD-40 protein family that was isolated as a binding partner of the extracellular signal-regulated kinase (ERK) pathway scaffold protein MP1. MORG1 specifically associates with several components of the ERK pathway, including MP1, Raf-1, MEK, and ERK, and stabilizes their assembly into an oligomeric complex. MORG1 facilitates ERK activation when cells are stimulated with lysophosphatidic acid, phorbol 12-myristate 13-acetate, or serum, but not in response to epidermal growth factor. Suppression of MORG1 by short interfering RNA leads to a marked reduction in ERK activity when cells are stimulated with serum. MORG1 is a component of a modular scaffold system that participates in the regulation of agonist-specific ERK signaling.

Spezifität: Human, mouse, rat. No cross reactivity with other proteins.

Anwendungen

Applikationshinweise: Western blotting: At 1u/ml with the appropriate system to detect MORG1 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 0.5-1u/ml to detect MORG1 in formalin fixed and paraffin embedded tissues. Immunocytochemistry Suitable Other applications have not been tested. Optimal dilutions should be determined by end user.

Reinheit: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Immunohistochemical analysis of paraffin-embedded Mammary cancer sectionsStaining MORG1 in cytoplasmDAB chromogenic reaction