BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) Antikörper

Antigen: BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3)

Synonyme: NIP3

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Western Blot (WB)

Produktnummer: ABIN343450

Produktbeschreibung

Weitere Bezeichnung: NIP3/BNIP3

Immunogen: A synthetic peptide corresponding to a sequence near the C-terminal of human NIP3, identical to the related mouse and rat sequence.

Format: Affinity-purified

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: The Bcl-2 nineteen kilodalton interacting protein 3 (BNIP3 or NIP3), is a hypoxia-inducible proapoptotic member of the Bcl-2 family that induces cell death by associating with the mitochondria. BNIP3, expressed in skeletal muscle and in the brain at low levels, is primarily localized to the nucleus of glial cells of the normal human brain, as well as in the malignant glioma cell line U251. BNIP3 expression in the cytoplasm increases and localizes with the mitochondria, contributing to induction of cell death. Cellular protein BNIP3 interacts with E1B-19K, BCL-2, BCL-xL, and EBV-BHRF1. BNIP3 contains Bcl-2 homology 3 (BH3) domain and COOH-terminal transmembrane (TM) domain. The BH3 domain of BNIP3 mediates Bcl-2/Bcl-X(L) heterodimerization and confers pro-apoptotic activity, whereas the TM domain is critical for homodimerization, pro-apoptotic function, and mitochondrial targeting.

Spezifität: Human, mouse, rat. No cross reactivity with other proteins.

Anwendungen

Applikationshinweise: Western blotting: At 1u/ml with the appropriate system to detect NIP3 in cells and tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Reinheit: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis of rat thymus tissue lysis using NIP3 antibody

Immunohistochemical analysis of paraffin-embedded Mammary cancer sections. Staining NIP3 in nucleusDAB chromogenic reaction