Enolase 2 (Gamma, Neuronal) (ENO2) Antikörper

Antigen: Enolase 2 (Gamma, Neuronal) (ENO2)

Synonyme: ENO2, DKFZp459B1817, NSE, Eno-2, AI837106, D6Ertd375e, RNEN3, Lrpb7

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Western Blot (WB), Immunhistochemie (Paraffinschnitte) (IHC (p)), Immunzytochemie (ICC)

Produktnummer: ABIN343446

Produktbeschreibung

Weitere Bezeichnung: Neuron specific enolase, NSE

Immunogen: A peptide mapping at the C-terminus of NSE of human origin, identical to the related mouse and rat sequence.

Format: Affinity-purified

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: NSE (neuron specific enolase), also known as Enolase 2(ENO2), is found in elevated concentrations in plasma in certain neoplasias. The enolases catalyze the interconversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. ENO2 gene contains 12 exons distributed over 9,213 nucleotides. Human neurone-specific enolase is mapped to chromosome 12p13.

Spezifität: Human, mouse, rat. No cross reactivity with other proteins.

Anwendungen

Applikationshinweise: Western blotting: At 1-2u/ml with the appropriate system to detect NSE in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2u/ml to detect NSE in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Immunocytochemistry Suitable Other applications have not been tested. Optimal dilutions should be determined by end user.

Reinheit: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis of rat brain tissue lysis using NSE antibody

Immunohistochemical analysis of paraffin-embedded Rat Tissue sections (brain)Using NSE antibody