Protein Phosphatase 2A Catalytic, PP2A Antikörper

Antigen: Protein Phosphatase 2A Catalytic, PP2A

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Western Blot (WB), Immunzytochemie (ICC), Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN343439

Produktbeschreibung

Immunogen: A synthetic peptide corresponding to a sequence mapping near the N-terminal of human PP2A, identical to the related rat and mouse sequence.

Format: Affinity-purified

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: The catalytic subunit of human protein phosphatase 2A (PPP2CA) encodes a 309-amino acid polypeptide.It is localized to chromosome 5. The gene (approximately 30 kbp) is composed of seven exons and six introns. It is predicted to be important for phosphatase enzymatic activity. Methylation of the C-terminal leucine residue (Leu309) of protein serine/threonine phosphatase 2A catalytic subunit (PP2AC) is known to regulate catalytic activity in vitro. Furthermore, PP2A has a fundamental role in cardiac function, and suggests that disturbances in protein phosphatase expression and activity may cause or exacerbate the course of cardiac diseases.

Spezifität: Human, mouse, rat. No cross reactivity with other proteins.

Anwendungen

Applikationshinweise: Western blotting: At 1-2u/ml with the appropriate system to detect PP2A in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2u/ml to detect PP2A in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Immunocytochemistry At 0.5-1u/ml to detect PP2A in acetone fixed cell. Antigen retrieval by Pepsin and Trypsin is required. Other applications have not been tested. Optimal dilutions should be determined by end user.

Reinheit: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis of rat kidney tissue lysis using PP2A antibody

Immunohistochemical analysis of paraffin-embedded Mammary cancer sections. Staining PP2A in cytoplasmDAB chromogenic reaction