Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2) Antikörper

Antigen: Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2)

Synonyme: ICH1, NEDD2, CASP-2, ICH-1L, ICH-1L/1S, ICH-1, Nedd2, Caspase-2, xCaspase-2

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Ratte (Rattus), Maus

Applikation: Western Blot (WB), Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN343415

Produktbeschreibung

Weitere Bezeichnung: Caspase 2

Immunogen: A synthetic peptide mapping at the C-terminal of human Caspase 2, identical to the related rat sequence.

Format: Affinity-purified

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Caspase-2, which is involved in stress-induced apoptosis, is recruited into a large protein complex, the molecular composition of which remains elusive. activation of caspase-2 occurs in a complex that contains the death domain-containing protein PIDD, whose expression is induced by p53, and the adaptor protein RAIDD. Increased PIDD expression resulted in spontaneous activation of caspase-2 and sensitization to apoptosis by genotoxic stimuli. Caspase-2 acts both as a positive and negative cell death effector, depending upon cell lineage and stage of development.

Spezifität: Human, rat, mouse. No cross reactivity with other proteins.

Anwendungen

Applikationshinweise: Western blotting: At 0.5-1u/ml with the appropriate system to detect Caspase 2 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2 u/ml to detect Caspase 2 in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Other applications have not been tested. Optimal dilutions should be determined by end user.

Reinheit: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Forschungsgebiet: Apoptose/Nekrose, Proteolyse / Ubiquitin

Beschränkungen: Nur für Forschungszwecke einsetzbar