Arylsulfatase D Antikörper (AA 108-120)

Produktnummer ABIN343011

Produktdetails anti-Arylsulfatase D Antikörper

Target: Arylsulfatase D (ARSD)

Bindungsspezifität: AA 108-120

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Arylsulfatase D Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB)

Spezifität: Human ARSD / Arylsulfatase D. This antibody is expected to recognise both reported isoforms (NP_001660.2, NP_033667.2)

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%) Marmoset (92%) Opossum, Platypus (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-DASNGYRALQWNA from an internal region of human ARSD / Arylsulfatase D (NP_001660.2, NP_033667.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%), Marmoset (92%), Opossum, Platypus (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: Peptide-ELISA (1:16000, 0.3 - 1 μg/mL), WB (0.3 - 1 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx 60+40kD bands observed in lysates of cell lines A549 and HepG2 (calculated MW of 64.9kD according to NP_001660.2 and of 42.3 according to NP_033667.2). Recommended concentration: 0.3-1 μg/mL. An additional band of unknown identity was also consistently observed at 35kD. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Arylsulfatase D

Target: Arylsulfatase D (ARSD)

Andere Bezeichnung: ARSD / Arylsulfatase D (ARSD Produkte)

Synonyme: ASD antikoerper, ARSD antikoerper, MGC81982 antikoerper, arylsulfatase D antikoerper, arylsulfatase D L homeolog antikoerper, ARSD antikoerper, arsd.L antikoerper, LOC516494 antikoerper, arsd antikoerper, BMEA_B1180 antikoerper, LOC100624032 antikoerper

Hintergrund: Name/Gene ID: ARSD

Synonyms: ARSD, Arylsulfatase D, ASD

Gen-ID: 414

NCBI Accession: NP_001660, NP_033667

UniProt: P51689