NR4A1 Antikörper (AA 401-414)

Produktnummer ABIN337182

Produktdetails anti-NR4A1 Antikörper

Target: NR4A1 (Nuclear Receptor Subfamily 4, Group A, Member 1 (NR4A1))

Bindungsspezifität: AA 401-414

Reaktivität: Human, Maus, Ratte, Hund, Pferd, Kaninchen, Hamster

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser NR4A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human NR4A1 / NUR77. This antibody is expected to recognise isoform a (NP_002126.2 and NP_775180.1) only.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Dog, Horse, Rabbit (100%) Marmoset, Bat (93%) Bovine (86%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-GRLPSKPKQPPDAS from an internal region of human NR4A1 / NUR77 (NP_002126.2, NP_775180.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Dog, Horse, Rabbit (100%), Marmoset, Bat (93%), Bovine (86%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu NR4A1

Target: NR4A1 (Nuclear Receptor Subfamily 4, Group A, Member 1 (NR4A1))

Andere Bezeichnung: NR4A1 / NUR77 (NR4A1 Produkte)

Synonyme: GFRP1 antikoerper, HMR antikoerper, N10 antikoerper, NAK-1 antikoerper, NGFIB antikoerper, NP10 antikoerper, NUR77 antikoerper, TR3 antikoerper, NGFI-B antikoerper, Gfrp antikoerper, Hbr-1 antikoerper, Hbr1 antikoerper, Hmr antikoerper, TIS1 antikoerper, nur77 antikoerper, Ngfi-b antikoerper, Nur77 antikoerper, nuclear receptor subfamily 4 group A member 1 antikoerper, nuclear receptor subfamily 4, group A, member 1 antikoerper, NR4A1 antikoerper, Nr4a1 antikoerper

Hintergrund: Name/Gene ID: NR4A1
Subfamily: NR4 Nerve growth factor-like
Family: NHR

Synonyms: NR4A1, GFRP1, Early response protein NAK1, HMR, NGFIB, Orphan nuclear receptor TR3, Orphan receptor tr3, Steroid receptor TR3, TR3, NAK1, NGFI-B, NP10, Receptor NGFIB, Testicular receptor 3, TR3 orphan receptor, N10, NAK-1, NUR77, Orphan nuclear receptor HMR

Gen-ID: 3164

NCBI Accession: NP_002126, NP_775180

UniProt: P22736

Pathways: Fc-epsilon Rezeptor Signalübertragung, Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Steroid Hormone Mediated Signaling Pathway