Occludin Antikörper (OCLN) (C-Term)

Details for Product anti-OCLN Antibody No. ABIN337048, Anbieter: Anmelden zum Anzeigen
Antigen
  • tpmt
  • OCLN
  • BLCPMG
  • AI503564
  • Ocl
  • ocln
  • oclnb
  • wu:fd23h10
  • wu:fi13c01
  • zgc:113992
  • zgc:56359
  • occludin
  • occludin a
  • OCLN
  • ocln
  • Ocln
  • ocln-b
  • oclna
Epitop
C-Term
33
15
13
7
7
6
5
5
4
4
3
3
2
2
1
1
Reaktivität
Hund, Human, Maus, Ratte (Rattus)
115
56
50
17
16
4
4
4
3
2
2
2
Wirt
Maus
87
32
3
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser Occludin Antikörper ist unkonjugiert
6
6
5
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
104
54
28
25
24
13
10
7
3
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Marke IHC-plus™
Immunogen GST fusion protein consisting of the C-terminal region (~150aa) of human occludin.

Type of Immunogen: Fusion protein
Klon OC-3F10
Isotyp Kappa, IgG1
Spezifität Recognizes mammalian Occludin Protein. Species cross-reactivity: human, mouse, rat and canine.
Reinigung Protein A purified
Andere Bezeichnung OCLN / Occludin (OCLN Antibody Abstract)
Hintergrund Name/Gene ID: OCLN

Synonyms: OCLN, Occludin, BLCPMG
Gen-ID 100506658
UniProt Q16625
Pathways Cell-Cell Junction Organization, Hepatitis C
Applikationshinweise Approved: ELISA (0.1 - 1 μg/mL), IF (2 - 3 μg/mL), IHC-P (10 μg/mL), WB (0.1 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Positive control: T84 cell line (human intestinal epithelium), MDCK cells (canine kidney), rat liver and Caco-2 cells (human colon adenocarcinoma). Does not appear to detect the most highly phosphorylated form of the occludin protein when tested on Caco-2 cells.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration Lot specific
Buffer PBS, pH 7.4, 0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeat freeze-thaw cycles.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.
Bilder des Herstellers
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Occludin (OCLN) (C-Term) antibody (ABIN337048) Human Kidney (formalin-fixed, paraffin-embedded) stained with OCLN antibody ABIN33704...
Produkt verwendet in: Krueger, Härtig, Reichenbach, Bechmann, Michalski: "Blood-brain barrier breakdown after embolic stroke in rats occurs without ultrastructural evidence for disrupting tight junctions." in: PLoS ONE, Vol. 8, Issue 2, pp. e56419, 2013 (PubMed). (1:200).

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