Nestin (NES) Antikörper
| Antigen |
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| Synonyme |
FLJ21841, Nbla00170, C78523, ESTM46, AA166324, NES, paranemin |
| Klonalität |
Monoklonal (10C2) |
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Wirt
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Reaktivität
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Human (86), Maus (37), Ratte (Rattus) (33), Rind (Kuh) (7), Kaninchen (7), Hund (6), Pferd (4), Schwein (4), Hamster (2), Huhn (1), Schimpanse (1), Affe (1), Schaf (1)
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Applikation
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 Alternativen Durchflusszytometrie (FACS), Immunhistochemie (IHC), Western Blot (WB)
Western Blot (WB) (70), Immunfluoreszenz (IF) (61), Durchflusszytometrie (FACS) (49), Immunhistochemie (Paraffinschnitte) (IHC (p)) (36), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (24), Immunpräzipitation (IP) (13), ELISA (12), Enzyme Immunoassay (EIA) (10), Immunhistochemie (IHC) (10), Immunzytochemie (ICC) (5), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), Immunoelectron Microscopy (IEM) (2)
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| Produktnummer |
ABIN335621 |
| Menge |
100 µg (Varianten) |
| Preis |
295,00 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 5 bis 7 Werktagen |
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Weitere Bezeichnung
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Nestin
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Format
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Affinity-purified
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Isotyp
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IgG1 (Passende Sekundärantikörper)
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Klon
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10C2
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Beschreibung
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The monoclonal antibody 10C2 recognizes human nestin (residues 14641614). Nestin is a 220240 kDa Class VI intermediate filament protein that is expressed in stem cells of the developing nervous system, specifically the neuroepithelium and angiogenic cells, and progenitors of pancreatic islet and mesenchymal cells. Additionally, expression has been identified in a variety of tumors such as glioblastomas and pancreatic tumors. Nestin containing filaments upon differentitation are replaced by cell type specific intermediate filament proteins, such as GFAP. No coreactivity to rodent nestins has been observed using 10C2 antibody.
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Applikationshinweise
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This 10C2 antibody has been tested by immunoblot analysis of cell line. This can be used at less than or equal to 5 ug per million cells in a 100 ul total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
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Buffer
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aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
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Lagerung
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Store at 4°C.Avoid repeated freeze/thaw cycles.
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "Nestin (NES)", Typ "Antikörper" finden
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Wirte
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Kaninchen (81), Maus (29), Huhn (3), Ziege (1)
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Reaktivitäten
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Human (86), Maus (37), Ratte (Rattus) (33), Rind (Kuh) (7), Kaninchen (7), Hund (6), Pferd (4), Schwein (4), Hamster (2), Huhn (1), Schimpanse (1), Affe (1), Schaf (1)
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Applikationen
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Western Blot (WB) (70), Immunfluoreszenz (IF) (61), Durchflusszytometrie (FACS) (49), Immunhistochemie (Paraffinschnitte) (IHC (p)) (36), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (24), Immunpräzipitation (IP) (13), ELISA (12), Enzyme Immunoassay (EIA) (10), Immunhistochemie (IHC) (10), Immunzytochemie (ICC) (5), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), Immunoelectron Microscopy (IEM) (2)
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Konjugate
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Biotin (4), HRP (4), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2)
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Epitope
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C-Term (4), AA 1484-1500 (1), AA 544-776 (1)
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