Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (Biliary Glycoprotein) (CEACAM1) Antikörper (Biotin)

Antigen: Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (Biliary Glycoprotein) (CEACAM1)

Synonyme: BGP, BGP1, BGPI, 2010110O04Rik, zeh0068, hm:zeh0068, CEACAM8, CEACAM1, MGC142545, Bgp, BGPR, CD66a, Ccam1

Klonalität: Monoklonal (CC1)

Wirt: Maus

Reaktivität: Maus

Konjugat: Biotin

Applikation: Durchflusszytometrie (FACS), Immunhistochemie (IHC)

Produktnummer: ABIN335547

Produktbeschreibung

Weitere Bezeichnung: CD66A

Isotyp: IgG1

Klon: CC1

Beschreibung: The monoclonal antibody CC1 recognizes CD66a, also known as carcinoembryonic antigen­related cell adhesion molecule 1 (CEACAM1), biliary glycoprotein, and BPG. Expression of CD66a is found on brush borders, epithelial, and endothelial cells. In hematopoietic cells expression is found abundantly on B cells, as well as some NKs, monocytes, DCs, and granulocytes. Although low levels of mRNA have been identified in T cells in humans, resting mouse T lymphocytes are not reported to express CD66a, as confirmed by lack of staining with CC1 antibody. In humans, expression levels of CD66a have been used to identify malignancies. CD66a plays a key role as a regulator of BCR activation of B lymphocytes. An alternate allele, CEACAM1b, is expressed in SJL mice? therefore, CC1 does not stain SJL tissue. The monoclonal CC1 has been shown to block viral infection and also enhance B cell proliferation when combined with IgM crosslinking.

Anwendungen

Applikationshinweise: This CC1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 ug per million cells in a 100 ul total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Buffer: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN 3

Lagerung: Store at 4°C.DO NOT FREEZE.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Staining of C57Bl/6 splenocytes with 0.03 ug of Biotin anti­mouse CD66a (CC1) followed by SAv­PE (cat. 12­4317) and Pacific Blue anti­ mouse CD3e (17A2) (cat. 57­0032)(left) or FITC anti­mo/hu B220 (RA3­6B2) (cat. 11­0452) (right). Quadrant lines denote isotype control levels. Total viable cells were used for analysis.