Cardiotin Antikörper

Antigen: Cardiotin

Klonalität: Monoklonal (SR-2)

Wirt: Maus

Reaktivität: Human, Schwein

Applikation: Immunhistochemie (IHC), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)

Produktnummer: ABIN335362

Produktbeschreibung

Immunogen: SR-2 is a mouse monoclonal IgM antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with the 100 kDa cardiotin subunit.

Format: Purified

Isotyp: IgM  (Passende Sekundärantikörper)

Klon: SR-2

Beschreibung: Cardiotin is a high molecular weight protein complex (300 kDa) located in the mitochondria of cardiomyocytes and skeletal muscle. The cardiotin structure exists of subunits of 60 kDa and 100 kDa, probably in a tetrameric configuration. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle alpha-actinin. During cardiac contractile dysfunction and myocard cell differentiation, the cardiotin distribution is affected. Compared to other structural proteins, cardiotin is one of the first to respond to insults (ischemia, fibrillation) that influence the functional status of cardiomyocytes.

Spezifität: Human and swine.

Anwendungen

Applikationshinweise: SR-2 reacts with cardiotin, a mitochondrion-associated protein, which is present in cardiomyocytes and skeletal muscle. SR-2 reacts with cardiomyocytes, skeletal muscle, stromal and epithelial cells as well in vivo as in vitro. SR-2 is useful for immunohistochemistry on frozen and paraffin-embedded tissue and immunoblotting. In immunoblotting assays SR-2 reacts with the 300 kDa cardiotin protein complex and its 100 kDa and 60 kDa subunits. Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:100 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:50 - 1:500 for immunoblotting applications.

Lagerung: Store at 4 o C, or in small aliquots at -20 o C.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-Cardiotin antibody

anti-Cardiotin antibody (Image 2)

Publikationen

Publikationen: Schaart, Moens, Endert et al.: "Biochemical characterization of cardiotin, a sarcoplasmic reticulum associated protein." in: FEBS letters, Vol. 403, Issue 2, pp. 168-72, 1997 (PubMed).