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Cardiotin Antikörper

Dieser Maus Monoklonal Antikörper detektiert spezifisch Cardiotin in WB, IHC (fro), IHC (p) und IHC. Es zeigt Reaktivität gegenüber Proben von Human und Schwein.Es wurde in 1 Publikation zitiert.
Produktnummer ABIN335362
544,85 €
Zzgl. Versandkosten 20,00 € und MwSt
0.1 mg
Lieferung nach: Deutschland
Lieferung in 2 bis 4 Werktagen

Kurzübersicht für Cardiotin Antikörper (ABIN335362)

Target

Cardiotin

Reaktivität

  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Human, Schwein

Wirt

  • 6
Maus

Klonalität

  • 6
Monoklonal

Konjugat

  • 6
Unkonjugiert

Applikation

Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Klon

SR-2
  • Spezifität

    Human and swine.

    Aufreinigung

    Purified

    Immunogen

    SR-2 is a mouse monoclonal IgM antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with the 100 kDa cardiotin subunit.

    Isotyp

    IgM
  • Applikationshinweise

    SR-2 reacts with cardiotin, a mitochondrion-associated protein, which is present in cardiomyocytes and skeletal muscle. SR-2 reacts with cardiomyocytes, skeletal muscle, stromal and epithelial cells as well in vivo as in vitro. SR-2 is useful for immunohistochemistry on frozen and paraffin-embedded tissue and immunoblotting. In immunoblotting assays SR-2 reacts with the 300 kDa cardiotin protein complex and its 100 kDa and 60 kDa subunits. Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:100 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:50 - 1:500 for immunoblotting applications.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    4 °C
  • Schaart, Moens, Endert, Ramaekers: "Biochemical characterization of cardiotin, a sarcoplasmic reticulum associated protein." in: FEBS letters, Vol. 403, Issue 2, pp. 168-72, (1997) (PubMed).

  • Target

    Cardiotin

    Hintergrund

    Cardiotin is a high molecular weight protein complex (300 kDa) located in the mitochondria of cardiomyocytes and skeletal muscle. The cardiotin structure exists of subunits of 60 kDa and 100 kDa, probably in a tetrameric configuration. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle alpha-actinin. During cardiac contractile dysfunction and myocard cell differentiation, the cardiotin distribution is affected. Compared to other structural proteins, cardiotin is one of the first to respond to insults (ischemia, fibrillation) that influence the functional status of cardiomyocytes.
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