NCAM / CD56 Antikörper

Antigen: NCAM / CD56

Klonalität: Monoklonal (RNL-1)

Wirt: Maus

Reaktivität: Human, Maus

Applikation: Durchflusszytometrie (FACS), Immunzytochemie (ICC), Immunhistochemie (IHC)

Produktnummer: ABIN335252

Produktbeschreibung

Immunogen: RNL-1 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with the small cell lung cancer cell line NCI-H82.

Format: Culture supernatant

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: RNL-1

Beschreibung: NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig)-like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins.

Spezifität: Human and mouse.

Anwendungen

Applikationshinweise: RNL-1 was defined as a cluster I antibody during the Second International Workshop on Small Cell Lung Cancer (SCLC) Antibodies. RNL-1 recognizes NCAM which is present in small cell lung cancer (SCLC) and lung carcinoids. RNL-1 is suitable for immunocytochemistry and immunohistochemistry on frozen tissues and for staining with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent Optimal antibody dilution should be determined by titration.

Lagerung: Store at 4 o C, or in small aliquots at -20 o C.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-NCAM / CD56 antibody

anti-NCAM / CD56 antibody (Image 2)

Publikationen

Publikationen: Mijnheere, Boerman, Broers et al.: "Immunotargeting of human small cell lung cancer xenografts in athymic mice using a monoclonal antibody (RNL-1) against a neuroendocrine-related antigen." in: The British journal of cancer. Supplement, Vol. 14, pp. 78-81, 1991 (PubMed).

Boerman, Mijnheere, Broers et al.: "Biodistribution of a monoclonal antibody (RNL-1) against the neural cell adhesion molecule (NCAM) in athymic mice bearing human small-cell lung-cancer xenografts." in: International journal of cancer. Journal international du cancer, Vol. 48, Issue 3, pp. 457-62, 1991 (PubMed).

Broers, Mijnheere, Rot et al.: "Novel antigens characteristic of neuroendocrine malignancies." in: Cancer, Vol. 67, Issue 3, pp. 619-33, 1991 (PubMed).

Hens, Nuydens, Geerts et al.: "Neuronal differentiation is accompanied by NSP-C expression." in: Cell and tissue research, Vol. 292, Issue 2, pp. 229-37, 1998 (PubMed).