Bromodeoxyuridine (BrDU) Antikörper

Antigen: Bromodeoxyuridine (BrDU)

Klonalität: Monoklonal (IIB5)

Wirt: Maus

Applikation: Durchflusszytometrie (FACS), Immunzytochemie (ICC), Immunhistochemie (IHC), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN335207

Produktbeschreibung

Weitere Bezeichnung: Bromodeoxyuridine / BrdU

Immunogen: IIB5 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse intraperitoneally immunized with BrdU conjugated to bovine serum albumin.

Format: Culture supernatant

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: IIB5

Beschreibung: The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.

Anwendungen

Applikationshinweise: IIB5 reacts with bromodeoxyuridine also when incorporated into nuclear DNA. IIB5 is suitable for flow cytometry and immunohistochemistry on frozen and paraffin-embedded tissues with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. Optimal antibody dilution should be determined by titration.

Lagerung: Store at 4 o C, or in small aliquots at -20 o C.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Schutte, Reynders, van Assche et al.: "An improved method for the immunocytochemical detection of bromodeoxyuridine labeled nuclei using flow cytometry." in: Cytometry, Vol. 8, Issue 4, pp. 372-6, 1987 (PubMed).

Tinnemans, Schutte, Lenders et al.: "Cytokinetic analysis of lung cancer by in vivo bromodeoxyuridine labelling." in: British journal of cancer, Vol. 67, Issue 6, pp. 1217-22, 1993 (PubMed).

van Engeland, Kuijpers, Ramaekers et al.: "Plasma membrane alterations and cytoskeletal changes in apoptosis." in: Experimental cell research, Vol. 235, Issue 2, pp. 421-30, 1997 (PubMed).

Schutte, Nieland, van Engeland et al.: "The effect of the cyclin-dependent kinase inhibitor olomoucine on cell cycle kinetics." in: Experimental cell research, Vol. 236, Issue 1, pp. Apr 15, 1997 (PubMed).