Hepatitis B Virus (AD & AY Antigens) Antikörper

Antigen: Hepatitis B Virus (AD & AY Antigens)

Klonalität: Monoklonal (NYRHepB)

Wirt: Maus

Applikation: ELISA

Produktnummer: ABIN334778

Produktbeschreibung

Immunogen: recombinant Hep B vaccine (BTG)

Isotyp: IgG1

Klon: NYRHepB

Beschreibung: Hepatitis B is one of a few known non-retroviral viruses which employ reverse transcription as a part of its replication process. (HIV, a completely unrelated virus, also uses reverse transcription, but it is a retrovirus.) HBV invades the cell by binding to surface receptor and become internalized. The viral core particles then migrate to the hepatocyte nucleus and the partially double-stranded, relaxed circular genomes (RC-DNA) are repaired to form a covalently closed circular DNA (cccDNA), which is the template for viral genomic and sub-genomic RNAs by cellular RNA polymerase II. Of these, the pregenomic RNA (pgRNA is selectively packaged into progeny capsids and is then reverse-transcribed into new RC-DNA. The core can either bud into the endoplasmic reticulum to be enveloped or exported from the cell or recycled back into the genome for conversion to cccDNA.

Anwendungen

Applikationshinweise: By direct ELISA (against r.hepatitis vaccine), 1:10,000 dilution will yield 0.5 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories). Additional Information: This antibody recognizes both the AD and the AY antigens in direct ELISA.

Konzentration: 1mg/ml in PBS (after reconstitution)

Reinigung: Ion exchange column

Lagerung: Shipping Condition: Antibody is shipped lyophilized at ambient temperature. Storage: In lyophilized form, for long periods, store at 4°C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20°C. Reconstitution: Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf Life: Two years lyophilized, one month in solution at 4°C.

Beschränkungen: Nur für Forschungszwecke einsetzbar