FLAG peptide Antikörper

Antigen: FLAG peptide

Klonalität: Monoklonal (NYRFLAG)

Wirt: Maus

Applikation: ELISA

Produktnummer: ABIN334774

Produktbeschreibung

Immunogen: Flag-conjugated proteins

Isotyp: IgG1 kappa  (Passende Sekundärantikörper)

Klon: NYRFLAG

Beschreibung: A fusion tag called FLAG consists of eight amino acids (AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site. FLAG is specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody. denoted as M1. binds the peptide in the presence of bivalent metal cations. preferably Ca(+). Elution is effected by chelating agents. Another strategy is competitive elution with excess of free Flag Peptide. The FLAG peptide purifies and detects recombinant fusion proteins. FLAG peptide is useful in Western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization. Flag peptide is a hydrophilic tag which significantly improves the detection and purification of recombinant fusion proteins.

Anwendungen

Applikationshinweise: By direct ELISA, 1:20,000 dilution will yield >1.0 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories).

Konzentration: 1mg/ml in PBS (after reconstitution)

Reinigung: Protein A & Ion exchange column

Lagerung: Shipping Condition: Antibody is shipped lyophilized at ambient temperature. Storage: In lyophilized form, for long periods, store at 4°C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20°C. Reconstitution: Reconstitute with H20 to get a 1 mg/ml concentration. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf Life: Two years lyophilized, one month in solution at 4°C.

Beschränkungen: Nur für Forschungszwecke einsetzbar