Proliferating Cell Nuclear Antigen (PCNA) Antikörper

Antigen: Proliferating Cell Nuclear Antigen (PCNA)

Synonyme: MGC8367, PCNAR, Pcna/cyclin

Klonalität: Monoklonal (16D10)

Wirt: Ratte

Reaktivität: Human, Maus, Ratte (Rattus), Hamster

Applikation: Western Blot (WB), Immunpräzipitation (IP), Immunfluoreszenz (IF)

Produktnummer: ABIN334654

Produktbeschreibung

Format: Liquid, Supernatant

Isotyp: IgG2b

Klon: 16D10

Beschreibung: Proliferating cell nuclear antigen (PCNA) is a homotrimeric ring-shaped protein that encircles the DNA and acts as a stationary loading platform for multiple, transiently interacting partners participating in various DNA transactions. This essential cellular component, originally characterized as a nuclear antigen of dividing cells, is evolutionary highly conserved from yeast to human. Within the eukaryotic cell, PCNA plays a key role in DNA replication, repair, cell cycle regulation, and post-replicative transactions like DNA methylation and chromatin remodeling. All these cellular processes are regulated by a complex network comprising cell cycle dependent changes in expression levels, dynamics, interactions, and localization of PCNA. Here we offer the first rat monoclonal antibody (MAb) against human PCNA, designated as PCNA 16D10. PCNA 16D10 is highly affine and specific MAb suited for ELISA, immunoblotting, immunoprecipitation, and immunofluorescence. The characteristic punctate staining of S phase cells allows the identification of proliferating cells and the monitoring of cell cycle progression

Spezifität: Reacts with Human, Mouse, Rat, Hamster. Predicted to react: Cow, Dog, Sheep (Due to sequence homology)

Anwendungen

Applikationshinweise: Western Blot: Used at a dilution of 1/2000. Detects a band of approximately 31 kDa (predicted molecular weight PCNA: 31 kDa). For immunoprecipitation, 2 ug of PCNA 16D10 antibody was used incubation for 1 h at RT. Immunofluorescence: HeLa cells 3,7% PFA fixed/ Methanol fixed. Used at a dilution of 1/2000. Secondary antibody: donkey-anti-rat-Alexa488 (1/400)

Konzentration: 1 mg/ml

Buffer: Liquid, hybridoma supernatant. The hybridoma was cultured in protein free medium. Tissue culture supernatant was concentrated by centrifugation using a 30 kD exclusion membrane to an antibody concentration of 1 mg/ml. Preservative: 0.01% Sodium Azide

Lagerung: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C. Store at +4 degree C short term (1-2 weeks). Avoid freeze / thaw cycles.

Forschungsgebiet: Proliferationsmarker

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

For Immunofluorescence (IF): HeLa cells 3,7% PFA fixed/ Methanol fixed. 16D10 used at a dilution of 1/2000. Secondary antibody: donkey-anti-rat-Alexa488 (1/400)

For Western Blot (WB): Used at a dilution of 1/2000. Detects a band of approximately 31 kDa (predicted molecular weight PCNA: 31 kDa).

For immunoprecipitation (IP): 2 µg of PCNA 16D10 antibody was used incubation for 1 h at RT

Publikationen

Publikationen: Spada, Haemmer, Kuch et al.: "DNMT1 but not its interaction with the replication machinery is required for maintenance of DNA methylation in human cells." in: The Journal of cell biology, Vol. 176, Issue 5, pp. 565-71, 2007 (PubMed).

Schermelleh, Haemmer, Spada et al.: "Dynamics of Dnmt1 interaction with the replication machinery and its role in postreplicative maintenance of DNA methylation." in: Nucleic acids research, Vol. 35, Issue 13, pp. 4301-12, 2007 (PubMed).

Rottach, Kremmer, Nowak et al.: "Generation and characterization of a rat monoclonal antibody specific for PCNA." in: Hybridoma (2005), Vol. 27, Issue 2, pp. 91-8, 2008 (PubMed).