Ubiquitin (UBQ11) Antikörper

Antigen: Ubiquitin (UBQ11)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Arabidopsis thaliana, Barley (Hordeum vulgare), Nicotiana benthamiana, Tomate (Solanum lycopersicum)

Applikation: Western Blot (WB)

Produktnummer: ABIN334561

Produktbeschreibung

Immunogen: recombinant Arabidopsis thaliana ubiquitin, ubq11, with N-terminal His-tag, locusaccession: At4g05050

Format: Lyophilized

Beschreibung: Ubiquitin is a highly conserved regulatory protein expressed in all eukaryotictissues. Originally this protein was called: Ubiquitous Immunopoietic Polypeptide.Its function is labeling of proteins for degradation through ubiquitin proteasomesystem (UPS).

Spezifität: Predicted reactivity: dicots including: Nicotiana tabacum, Glycine max, monocots including: Hordeumvulgare, Oryza sativa, Zea mays. Not reactive in: algae.

Anwendungen

Applikationshinweise: Recommended Dilution 1: 10 000 with standard ECL (WB).

Reinheit: Serum

Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

5 μg of recombinant protein from Phytophora sp. (1), human (2), Arabidopsis thaliana His-tagged ubiquitin (3), Arabidopsis thaliana His-tagged SUMO protein (4), was separated on 15% PAA gel and blotted on PVDF membrane. Filters were blocked in 5% milk for 1h, incubated with 1: 10 000 anti-ubiquitin antibody (1h), followed by incubation with 1: 15 000 secondary anti-rabbit antibodies(1h) coupled with HRP and visualization (10 seconds exposure) with standard ECL.