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Antikörper
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anti-Roundabout, Axon Guidance Receptor, Homolog 2 (Drosophila) (ROBO2) Antikörper
Roundabout, Axon Guidance Receptor, Homolog 2 (Drosophila) (ROBO2) Antikörper
| Antigen |
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| Synonyme |
SAX3, KIAA1568, ROBO2 |
| Klonalität |
Polyklonal |
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Wirt
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Applikation
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Durchflusszytometrie (FACS) (17), Immunfluoreszenz (IF) (16), Western Blot (WB) (11), Enzyme Immunoassay (EIA) (6), ELISA (5), Immunhistochemie (Paraffinschnitte) (IHC (p)) (4), Dot Blot (Dot) (1), ELISA (Detektionsantikörper) (1), Immunoelectron Microscopy (IEM) (1), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (1), Immunhistochemie (IHC) (1), Immunpräzipitation (IP) (1)
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| Produktnummer |
ABIN334482 |
| Menge |
100 ug |
| Preis |
184,00 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 5 Werktagen |
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Weitere Bezeichnung
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ROBO2
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Gen-ID
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6092, 268902 (mouse), 84409 (rat)
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Immunogen
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C-NQSQRPRPTKKHK
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Beschreibung
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All names and symbols: ROBO2, roundabout, axon guidance receptor, homolog 2 (Drosophila), KIAA1568, SAX3, roundabout, axon guidance receptor, homolog 2. Official Symbol: ROBO2. RefSeq number(s): NP_002933.1
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Spezifität
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Human, Mouse, Rat, Dog
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Applikationshinweise
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Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments gave an approx. 75kDa band in Human Brain (Amygdala) lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 151kDa according to NP_002933.1. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
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Reinigung
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Buffer
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Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Lagerung
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Aliquot and store at -20°C. Minimize freezing and thawing.
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Publikationen
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Fujisawa, Wrana, Culotti: "The slit receptor EVA-1 coactivates a SAX-3/Robo mediated guidance signal in C. elegans." in: Science (New York, N.Y.), Vol. 317, Issue 5846, pp. 1934-8, 2007 (PubMed).
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Alternativen zu Antigen "Roundabout, Axon Guidance Receptor, Homolog 2 (Drosophila) (ROBO2)", Typ "Antikörper" finden
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Wirte
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Kaninchen (25), Maus (5), Ziege (4)
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Reaktivitäten
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Human (30), Maus (23), Ratte (Rattus) (23), Hund (12), Rind (Kuh) (8), Pferd (7), Kaninchen (5), Schwein (3), Schaf (3), Huhn (2)
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Applikationen
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Durchflusszytometrie (FACS) (17), Immunfluoreszenz (IF) (16), Western Blot (WB) (11), Enzyme Immunoassay (EIA) (6), ELISA (5), Immunhistochemie (Paraffinschnitte) (IHC (p)) (4), Dot Blot (Dot) (1), ELISA (Detektionsantikörper) (1), Immunoelectron Microscopy (IEM) (1), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (1), Immunhistochemie (IHC) (1), Immunpräzipitation (IP) (1)
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Konjugate
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Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Alternativen 
