LAG1 Homolog, Ceramide Synthase 5/6 (LASS5/6) Antikörper

Antigen: LAG1 Homolog, Ceramide Synthase 5/6 (LASS5/6)

Synonyme: Trh4, CerS5, FLJ25304, MGC45411, LASS5, trh4, MGC159488

Klonalität: Polyklonal

Wirt: Ziege

Applikation: ELISA

Produktnummer: ABIN334429

Produktbeschreibung

Weitere Bezeichnung: LASS5+6 / CerS5+6

Gen-ID: 91012, 25, 71949 (mouse)

Immunogen: C-RGKVSKDDRSDVES

Beschreibung: All names and symbols: LASS5, LAG1 homolog, ceramide synthase 5, CerS5, FLJ25304, MGC45411, Trh4, LAG1 longevity assurance homolog 5. Official Symbol: LASS5. RefSeq number(s): NP_671723.1, NP_982288.1

Spezifität: This antibbody is expected to cross-react with the highly similar LASS6. Human, Mouse, Rat, Dog, Cow

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Preliminary experiments gave an approx. 60kDa band in lysates of HEK293, HeLa and HepG2 after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 45.8kDa according to NP_671723.1. The 60kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Min, Mesika, Sivaguru et al.: "(Dihydro)ceramide synthase 1 regulated sensitivity to cisplatin is associated with the activation of p38 mitogen-activated protein kinase and is abrogated by sphingosine kinase 1." in: Molecular cancer research : MCR, Vol. 5, Issue 8, pp. 801-12, 2007 (PubMed).