Fascin Homolog 2, Actin-Bundling Protein, Retinal (Strongylocentrotus Purpuratus) (FSCN2) Antikörper

Antigen: Fascin Homolog 2, Actin-Bundling Protein, Retinal (Strongylocentrotus Purpuratus) (FSCN2)

Synonyme: RFSN, RP30

Klonalität: Polyklonal

Wirt: Ziege

Applikation: ELISA

Produktnummer: ABIN334397

Produktbeschreibung

Weitere Bezeichnung: Fascin 2

Gen-ID: 25794, 238021 (mouse), 303741 (rat)

Immunogen: CHHRGSNQLDTNR

Beschreibung: All names and symbols: fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus), RFSN, RP30, fascin 2, retinal fascin,. Official Symbol: FSCN2 . RefSeq number(s): NP_001070650.1, NP_036550.1

Spezifität: This antibody is expected to recognize both reported isoforms (NP_001070650.1 and NP_036550.1). Human, Mouse, Rat, Dog

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave bands at approx 75kDa and 22kDa in Mouse Eye lysates after 0.05µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 57.4kDa according to NP_001070650.1 and 55.1kDa according to NP_036550.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Forschungsgebiet: Neurologie, Zytoskelett, Mikrofilamente

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Vignjevic, Kojima, Aratyn et al.: "Role of fascin in filopodial protrusion." in: The Journal of cell biology, Vol. 174, Issue 6, pp. 863-75, 2006 (PubMed).