HCST Antikörper (Hematopoietic Cell Signal Transducer) (C-Term)

Details for Product anti-HCST Antibody No. ABIN334384, Anbieter: Anmelden zum Anzeigen
Antigen
  • HCST
  • DAP10
  • KAP10
  • PIK3AP
  • Dap10
  • hematopoietic cell signal transducer
  • HCST
  • hcst
  • Hcst
Epitop
C-Term
12
2
1
1
1
Reaktivität
Human
30
1
1
1
Wirt
Ziege
15
15
Klonalität
Polyklonal
Konjugat
Dieser HCST Antikörper ist unkonjugiert
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Methodentyp
Peptide ELISA
Applikation
ELISA, Western Blotting (WB)
15
13
12
3
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Immunogen Peptide with sequence C-RSPAQEDGKVYIN, from the C Terminus of the protein sequence according to NP_055081.1, NP_001007470.1.
Blocking Peptide Zu diesem Produkt passt das Blocking Peptide ABIN369830.
Spezifität This antibody is expected to recognise isoform 1 (NP_055081.1) but it may recognize isoform 2 lacking residue E81 (NP_001007470.1).
Kreuzreaktivität Maus, Ratte (Rattus)
Reinigung Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Andere Bezeichnung DAP10 / HCST (HCST Antibody Abstract)
Hintergrund Synonyms: HCST, DAP10, hematopoietic cell signal transducer, KAP10, PIK3AP, DNAX-activation protein 10, phosphoinositide-3-kinase adaptor protein
Gen-ID 10870, 23900, 474146
NCBI Accession NP_001007470, NP_055081
Applikationshinweise ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 40 kDa band observed in human spleen lysates (calculated MW of 9.5 kDa according to NP_055081.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Wu et al, Science. 1999 Jul 30,285(5428):730-2., PMID: 10426994). Recommended concentration: 0.2-0.6 µg/mL. An additional band of unknown identity was also consistently observed at 100 kDa. This band was successfully blocked by incubation with the immunising peptide.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Minimize freezing and thawing.
Lagerung -20 °C
Informationen zur Lagerung Aliquot and store at -20 °C.
Bilder des Herstellers
 image for anti-Hematopoietic Cell Signal Transducer (HCST) (C-Term) antibody (ABIN334384) ABIN334384 (0.2µg/ml) staining of human spleen lysate (35µg protein in RIPA buffer). ...
Allgemeine Veröffentlichungen Upshaw, Arneson, Schoon, Dick, Billadeau, Leibson: "NKG2D-mediated signaling requires a DAP10-bound Grb2-Vav1 intermediate and phosphatidylinositol-3-kinase in human natural killer cells." in: Nature immunology, Vol. 7, Issue 5, pp. 524-32, 2006 (PubMed).

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