Retinoblastoma 1 Antikörper (pThr821)

Produktnummer ABIN3201013

Produktdetails anti-Retinoblastoma 1 Antikörper

Target: Retinoblastoma 1 (RB1)

Bindungsspezifität: pThr821

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Retinoblastoma 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Spezifität: Specific to human Rb phosphorylated at Thr821.

Kreuzreaktivität (Details): Not tested for other species. Can detect endogenous levels of Rb phosphorylated at Thr821 in most cell lines.

Aufreinigung: Purified

Sterilität: Sterile filtered

Immunogen: A synthetic peptide containing pThr821 of human Rb

Klon: 24A7

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: 1. Western blotting: ~1 g/mL
2. ELISA
Other applications were not tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, 50 % glycerol

Konservierungsmittel: Azide free

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 C (For long term storage: -70 C).

Referenzen für anti-Retinoblastoma 1 Antikörper (ABIN3201013)

Sherr: "Cancer cell cycles." in: Science (New York, N.Y.), Vol. 274, Issue 5293, pp. 1672-7, (1997) (PubMed).

Welch, Wang: "A C-terminal protein-binding domain in the retinoblastoma protein regulates nuclear c-Abl tyrosine kinase in the cell cycle." in: Cell, Vol. 75, Issue 4, pp. 779-90, (1993) (PubMed).

Nevins: "E2F: a link between the Rb tumor suppressor protein and viral oncoproteins." in: Science (New York, N.Y.), Vol. 258, Issue 5081, pp. 424-9, (1992) (PubMed).

Hu, Dyson, Harlow: "The regions of the retinoblastoma protein needed for binding to adenovirus E1A or SV40 large T antigen are common sites for mutations." in: The EMBO journal, Vol. 9, Issue 4, pp. 1147-55, (1990) (PubMed).

Details zu Retinoblastoma 1

Target: Retinoblastoma 1 (RB1)

Andere Bezeichnung: RB (RB1 Produkte)

Synonyme: OSRC antikoerper, RB antikoerper, p105-Rb antikoerper, pRb antikoerper, pp110 antikoerper, Rb antikoerper, Rb-1 antikoerper, P105-RB antikoerper, PP110 antikoerper, RB11 antikoerper, zgc:154147 antikoerper, RB transcriptional corepressor 1 antikoerper, RB transcriptional corepressor like 1 antikoerper, retinoblastoma 1 antikoerper, RB1 antikoerper, RBL1 antikoerper, Rb1 antikoerper, rb1 antikoerper

Hintergrund: Retinoblastoma protein (Rb), the tumor suppressor product of the retinoblastoma susceptibility gene, is a 110 kDa protein that functions as a negative regulator of the cell cycle by arresting cells in the G1 phase and halting inappropriate cell proliferation. At the transcriptional level, Rb protein exerts its growth suppressive function by binding to transcription factors including E2F1, PU1, ATF2, UBF, Elf-1 and c-Abl. Loss of Rb function leads to uncontrolled cell growth and tumor development and is found in all retinoblastomas and in a variety of other human malignancies. The ability of Rb protein to inhinbit transcription and cell cycle progression is inactivated by hosphorylation, which is catalyzed by the cyclin-dependent protein kinases.

UniProt: P06400

Pathways: Zellzyklus, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagie