Dieser MDM4-binding Protein Antikörper ist unkonjugiert
Applikation
Western Blotting (WB), ELISA, Immunoprecipitation (IP)
Produktmerkmale
Product: Mouse monoclonal antibody (clone #15) specific to the MdmX protein phosphorylated at Ser367, Human and mouse MdmX proteins phosphorylated at Ser367
Aufreinigung
Purified
Immunogen
A synthetic peptide corresponding to a sequence of human Mdmx protein surrounding pSer367
Chen, Gilkes, Pan, Lane, Chen: "ATM and Chk2-dependent phosphorylation of MDMX contribute to p53 activation after DNA damage." in: The EMBO journal, Vol. 24, Issue 19, pp. 3411-22, (2005) (PubMed).
Okamoto, Kashima, Pereg, Ishida, Yamazaki, Nota, Teunisse, Migliorini, Kitabayashi, Marine, Prives, Shiloh, Jochemsen, Taya: "DNA damage-induced phosphorylation of MdmX at serine 367 activates p53 by targeting MdmX for Mdm2-dependent degradation." in: Molecular and cellular biology, Vol. 25, Issue 21, pp. 9608-20, (2005) (PubMed).
Target
MDM4-binding Protein (MDM4)
(Mdm4-binding Protein (MDM4))
MdmX (synonyms: Mdm4, HdmX) inhibits p53-and p73-dependent cell cycle arrest and apoptosis by binding to the transcription activation domains of these proteins. MdmX consists of 490 amino acids with the molecular weight of 54,864 and contains a RIING-finger domain and a nuclear transport signal. It is known that the protein migrates aberrantly in SDS-PAGE at the position of an 80- kDa protein. MdmX is phosphorylated at Ser367 by Chk2 kinase downstream of ATM in response to DNA damage, and as a result, it binds to14-3-3 and is transported into nucleus where it is degraded by Mdm2. This process activates the p53 functions.