p53 Antikörper (pSer46)

Produktnummer ABIN3201005

Produktdetails anti-p53 Antikörper

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Bindungsspezifität: pSer46

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser p53 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Kreuzreaktivität (Details): Not tested in other species.

Aufreinigung: Purified from serum-free culture medium of hybridoma (#36) by proprietary chromatography procedures under mild conditions.

Sterilität: Sterile filtered

Immunogen: Synthetic peptide containing pSer46 of p53

Klon: 36

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: 1) Western blotting: 1,000~2,000 dilution
2) Immunohistochemistry (assay dependent)
3) ELISA
Other applications have not been tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS (x1), 50 % glycerol. Azide and carrier free.

Konservierungsmittel: Azide free

Lagerung: -20 °C

Informationen zur Lagerung: Upon arrival centrifuge briefly and store at -20 C.

Referenzen für anti-p53 Antikörper (ABIN3201005)

Taira, Yamamoto, Yamaguchi, Miki, Yoshida: "ATM augments nuclear stabilization of DYRK2 by inhibiting MDM2 in the apoptotic response to DNA damage." in: The Journal of biological chemistry, Vol. 285, Issue 7, pp. 4909-19, (2010) (PubMed).

Kodama, Otsubo, Hirota, Yokota, Enari, Taya: "Requirement of ATM for rapid p53 phosphorylation at Ser46 without Ser/Thr-Gln sequences." in: Molecular and cellular biology, Vol. 30, Issue 7, pp. 1620-33, (2010) (PubMed).

Taira, Nihira, Yamaguchi, Miki, Yoshida: "DYRK2 is targeted to the nucleus and controls p53 via Ser46 phosphorylation in the apoptotic response to DNA damage." in: Molecular cell, Vol. 25, Issue 5, pp. 725-38, (2007) (PubMed).

Details zu p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Andere Bezeichnung: p53 (TP53 Produkte)

Hintergrund: P53 mutants are found in more than half of human cancers and are considered as the most important human cancer related gene. p53 is detected at 53kD position by electrophoresis and is composed of 393 amino acids. In the unstressed normal cells the p53 level is low and it is inactive. However, with stress, especially with DNA damage, it is activated to promote arrest of cell cycle and repair of DNA damage, or induction of apoptosis. The functions of p53 are regulated by phosphorylation of serine and threonine, and acetylation of lysine at various sites in the molecule. Among the phosphorylation sites, Ser46 is phosphorylated when DNA damage is so severe as to become unrepairable, which leads to apoptosis by activating transcription of proapoptotic genes such as p53AIPI As to the kinase of phosphorylation of Ser46, involvement of DYRK2 and ATM have been implicated.

UniProt: P04637

Pathways: p53 Signalweg, MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagie, Warburg Effekt