ATP6V0D2 Antikörper (Isoform 1)

Produktnummer ABIN3197488

Produktdetails anti-ATP6V0D2 Antikörper

Target: ATP6V0D2 (ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2))

Bindungsspezifität: Isoform 1

Reaktivität: Tomato, Arabidopsis thaliana, Pteris vittata (Chinese ladder brake), Mais (Zea mays), Gerste, Chlamydomonas reinhardtii, Lilium longiflorum (Trumpet lily), Oryza sativa

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATP6V0D2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Kreuzreaktivität (Details): No cross-reactivity with: mangrove plants,

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 26 / 31 kDa (Arabidopsis thaliana)

Aufreinigung: serum

Immunogen: KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase including Arabidopsis thaliana At4g11150. Peptide is conserved in vacuolar H+-ATPase subunit E, isoform 1 to 3 (VHA-E1).

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: 1 : 2 000 - 1 : 5000 with alkaline phosphatase (WB), 1 : 50 (IHC).
Cellular [compartment marker] of tonoplast membrane

Kommentare:

V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample.Immunostaining protocol using V-ATPase antibodies can be found here.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 10 µL of sterile water.

Handhabung: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Lagerung: -20 °C

Referenzen für anti-ATP6V0D2 Antikörper (ABIN3197488)

Bigeard, Rayapuram, Bonhomme, Hirt, Pflieger: "Proteomic and phosphoproteomic analyses of chromatin-associated proteins from Arabidopsis thaliana." in: Proteomics, Vol. 14, Issue 19, pp. 2141-55, (2014) (PubMed).

Schnell, Han, Miki, Johnson: "Soybean peroxidase propeptides are functional signal peptides and increase the yield of a foreign protein." in: Plant cell reports, (2010) (PubMed).

Hinton, Sennoune, Bond, Fang, Reuveni, Sahagian, Jay, Martinez-Zaguilan, Forgac: "Function of a subunit isoforms of the V-ATPase in pH homeostasis and in vitro invasion of MDA-MB231 human breast cancer cells." in: The Journal of biological chemistry, Vol. 284, Issue 24, pp. 16400-8, (2009) (PubMed).

Details zu ATP6V0D2

Target: ATP6V0D2 (ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2))

Andere Bezeichnung: V-ATPase (ATP6V0D2 Produkte)

Synonyme: ATP6D2 antikoerper, VMA6 antikoerper, AI324824 antikoerper, V-ATPase antikoerper, 1620401A02Rik antikoerper, ATPase H+ transporting V0 subunit d2 antikoerper, ATPase, H+ transporting, lysosomal V0 subunit D2 antikoerper, ATPase H+ transporting V0 subunit D2 antikoerper, ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d2 L homeolog antikoerper, ATP6V0D2 antikoerper, Atp6v0d2 antikoerper, atp6v0d2.L antikoerper

Hintergrund: AGI Code: At4g11150
Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448

Molekulargewicht: expected: 26 kDa, apparent: 31 kDa (Arabidopsis thaliana)

UniProt: Q39258

Pathways: Transition Metal Ion Homeostasis, Proton Transport