SMAD1 Antikörper (pSer465)

Produktnummer ABIN319257

Produktdetails anti-SMAD1 Antikörper

Target: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Bindungsspezifität: pSer465

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMAD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Spezifität: SMAD1 (Phospho-Ser465) Antibody AP08024PU detects endogenous levels of Smad1 only when phosphorylated at Serine 465.

Aufreinigung: Immunoaffinity Chromatography: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from Human Smad1 around the phosphorylation site of Serine 465 (I-S-S-V-SP).

Anwendungsinformationen

Applikationshinweise: Wetern Blot (1/500-1/1000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at-20 °C.

Details zu SMAD1

Target: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Andere Bezeichnung: SMAD1 (SMAD1 Produkte)

Synonyme: MLP antikoerper, Xmad1 antikoerper, Xsmad1 antikoerper, bsp1 antikoerper, jv4-1 antikoerper, jv41 antikoerper, madh1 antikoerper, madr1 antikoerper, smad-1 antikoerper, MADH1 antikoerper, fb39h09 antikoerper, wu:fb39h09 antikoerper, wu:fb97e04 antikoerper, 2/23 antikoerper, CG12399 antikoerper, Dmel\\CG12399 antikoerper, E(zen)2 antikoerper, En(vvl) antikoerper, MAD antikoerper, Mat antikoerper, P-Mad antikoerper, P-mad antikoerper, PMad antikoerper, Smad antikoerper, Smad1 antikoerper, apg antikoerper, c28 antikoerper, dMAD antikoerper, dMad antikoerper, l(2)K00237 antikoerper, l(2)k00237 antikoerper, mad antikoerper, p-Mad antikoerper, pMAD antikoerper, pMad antikoerper, pSmad antikoerper, BSP-1 antikoerper, BSP1 antikoerper, JV4-1 antikoerper, JV41 antikoerper, MADR1 antikoerper, AI528653 antikoerper, Madh1 antikoerper, Madr1 antikoerper, Mlp1 antikoerper, MusMLP antikoerper, smad1 antikoerper, xmad antikoerper, SMAD family member 1 antikoerper, Mothers against dpp antikoerper, SMAD family member 1 L homeolog antikoerper, smad1 antikoerper, SMAD1 antikoerper, Mad antikoerper, Smad1 antikoerper, smad1.L antikoerper

Hintergrund: SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD1, as a transcriptional modulator, is activated by BMP (Bone Morphogenetic Protein) type 1 receptor kinase (it is a receptor-regulated SMAD or R-SMAD). BMPs are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. SMAD proteins have been implicated as downstream effectors of TGF beta/BMP signaling. In response to BMP ligands, SMAD1 can be phosphorylated (other sites besides the most prominent of S206, are S187, S195, and S214). S-206 is phosphorylated by ERK in response to mitogenic growth factors, or by recombinant ERK in vitro, this can be tested by treating cells with EGF or in cancer cells where Ras is activated. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is also a target for SMADspecific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation.Synonyms: BSP-1, BSP1, JV4-1, MAD homolog 1, MADH1, MADR1, Mad-related protein 1, Mothers against DPP homolog 1, Mothers against decapentaplegic homolog 1, SMAD 1, SMAD family member 1, SMAD-1, Transforming growth factor-beta-signaling protein 1

Gen-ID: 4086

NCBI Accession: NP_001003688

UniProt: Q15797

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development