14-3-3 Pan Antikörper

Antigen: 14-3-3 Pan

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Rind (Kuh)

Applikation: Western Blot (WB)

Produktnummer: ABIN318952

Produktbeschreibung

Produktmerkmale: Synonyms: YWHA, Protein kinase C inhibitor protein 1

Weitere Bezeichnung: 14-3-3 protein pan

Immunogen: Peptide corresponding to amino acid residues from the C-terminal region of the 14-3-3protein, conjugated to keyhole limpet hemocyanin (KLH).

Kreuzreaktivität: Rind (Kuh), Hund, Huhn, Human, Affe, Maus, Ratte (Rattus), Xenopus laevis

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: 14-3-3 proteins form homo- and heterodimers that can interact with a variety of cellularproteins that are typically phosphorylated on serine or threonine residues. 14-3-3 proteinsregulate cellular processes such as trafficking, cell-cycle regulatory pathways and signaltransduction. Brain tissue contains the highest concentration of 14-3-3 proteins and theproteins have been found in the cerebral spinal fluid of patients with certainneurodegenerative disease such as Creutzfeldt-Jakob disease and scrapie. 14-3-3 proteinsmight also contribute to Alzheimers and Parkinsons diseases, MillerDieker syndrome andspinocerebellar ataxia type 1. 14-3-3 has also been implicated in cancer.

Spezifität: This antibody recognises 14-3-3 protein, a highly conserved, ubiquitously expressedprotein family. Species: Rat. Others not tested.

Anwendungen

Applikationshinweise: Western Blot: 1: 1000, detects a band of approximately 29kDa in rat hippocampal lysate. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Reinigung: Purified

Buffer: 10mM Hepes pH7.5, 150mM NaCl containing 0.09% Sodium Azide (NaN3),50% Glycerol 0.01% Bovine Serum Albumin

Lagerung: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Caution: (A full Health and Safety assessment is available upon request) This product containsSodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled bytrained staff only.

Forschungsgebiet: Transkriptionsfaktoren

Beschränkungen: Nur für Forschungszwecke einsetzbar