This antibody detects murine Mdm2, a nuclear phosphoprotein that controls intracellular levels of the tumour suppressor protein p53 by ubiquitination and subsequent proteasomal degradation.
Aufreinigung
Purified
Immunogen
Synthetic peptide corresponding to aa 177-195 of mouse Mdm2.
ELISA: 1: 3000 - 1: 12000. Western Blot: 1: 500 - 1: 2000, detects a band of approximately 90 kDa in mouse MEF celllysates. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Mdm2 also interacts with the retinoblastoma (RB) protein and E2F-1, to promote G1-S phase transition in the cell cycle. Mdm2 activity is controlled by self-ubiquitination, however activation of the PI3K/PKB pathway leads to phosphorylation of Mdm2 at Ser185, which stabilises the protein.Synonyms: Double minute 2 protein, E3 ubiquitin-protein ligase Mdm2, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2