Cullin 1 (CUL1) Antikörper
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| Synonyme |
LOC100282292, CUL1, Cullin-1, DKFZp459I053, MGC149834, MGC149835, Cul1, zgc:55747, zgc:77500, wu:fc13a10 |
| Klonalität |
Polyklonal |
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Wirt
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Reaktivität
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Applikation
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 Alternativen Enzyme Immunoassay (EIA), Immunpräzipitation (IP), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)
Western Blot (WB) (28), Immunfluoreszenz (IF) (21), Durchflusszytometrie (FACS) (19), Immunhistochemie (Paraffinschnitte) (IHC (p)) (19), Immunpräzipitation (IP) (16), ELISA (7), Enzyme Immunoassay (EIA) (3), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (2), Dot Blot (Dot) (1), ELISA (Detektionsantikörper) (1), Immunoelectron Microscopy (IEM) (1), Immunhistochemie (IHC) (1)
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| Produktnummer |
ABIN315535 |
| Menge |
50 µl (Varianten) |
| Preis |
410,00 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 7 bis 10 Werktagen |
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Weitere Bezeichnung
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Cullin 1
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Gen-ID
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8454, 26965
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Swiss-Prot
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Q13616
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Immunogen
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Synthetic peptide.
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Beschreibung
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Cullins assemble a potentially large number of ubiquitin ligases by binding to the RINGprotein ROC1 to catalyse polyubiquitination, as well as binding to various specificityfactors to recruit substrates. Cullin 1 is an essential component of the SCF(SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates theubiquitination of proteins involved in cell cycle progression, signal transduction andtranscription. In the SCF complex, cul1 serves as a rigid scaffold that organizes theSKP1-F-box protein and RBX1 subunits. Cul1 may also contribute to catalysis throughpositioning of the substrate and the ubiquitin-conjugating enzyme. Cul1 is part of theSCF complex consisting of CUL1, RBX1, SKP1 and SKP2, where it interacts directlywith SKP1, SKP2 and RBX1. Cul1 also interacts with RNF7 and is part of a complexwith TIP120A/CAND1 and RBX1. The unneddylated form interacts withTIP120A/CAND1 and the interaction negatively regulates the association with SKP1 inthe SCF complex. Alternate Names: anti-CUL1 antibody, CUL-1 antibody, CULLIN-1 antibody.
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Spezifität
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Amino acids 742-752 of Human Cul1 (C-terminus) coupled to KLH. 766-776. Species Reactivity: This antibody is reactive to Human, Mouse.
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Applikationshinweise
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Suggested working dilutions: Western Blot 1:500-1:1000,ELISA 1:2000-1:10000,Immunohistochemistry-Paraffin 1:500
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Buffer
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PBS
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Lagerung
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Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.
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Forschungsgebiet
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Apoptose/Nekrose, Proteasome
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "Cullin 1 (CUL1)", Typ "Antikörper" finden
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Wirte
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Kaninchen (46), Maus (2)
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Reaktivitäten
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Human (44), Maus (35), Ratte (Rattus) (22), Rind (Kuh) (2), Hund (2), Huhn (1), Pferd (1), Affe (1), Schwein (1), Kaninchen (1)
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Applikationen
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Western Blot (WB) (28), Immunfluoreszenz (IF) (21), Durchflusszytometrie (FACS) (19), Immunhistochemie (Paraffinschnitte) (IHC (p)) (19), Immunpräzipitation (IP) (16), ELISA (7), Enzyme Immunoassay (EIA) (3), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (2), Dot Blot (Dot) (1), ELISA (Detektionsantikörper) (1), Immunoelectron Microscopy (IEM) (1), Immunhistochemie (IHC) (1)
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Konjugate
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitope
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C-Term (2), C-Term AA 766-776 (1)
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