Nestin (NES) Antikörper
| Antigen |
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| Synonyme |
FLJ21841, Nbla00170, C78523, ESTM46, AA166324, NES, paranemin |
| Klonalität |
Polyklonal |
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Wirt
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Reaktivität
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 Alternativen Rind (Kuh), Hund, Hamster, Human, Maus, Kaninchen, Ratte (Rattus)
Human (86), Maus (36), Ratte (Rattus) (32), Rind (Kuh) (6), Kaninchen (6), Hund (5), Pferd (4), Schwein (4), Huhn (1), Schimpanse (1), Hamster (1), Affe (1), Schaf (1)
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Applikation
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Alternativen Enzyme Immunoassay (EIA), Immunfluoreszenz (IF), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)
Western Blot (WB) (70), Immunfluoreszenz (IF) (60), Durchflusszytometrie (FACS) (50), Immunhistochemie (Paraffinschnitte) (IHC (p)) (35), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (24), Immunpräzipitation (IP) (13), ELISA (12), Immunhistochemie (IHC) (11), Enzyme Immunoassay (EIA) (9), Immunzytochemie (ICC) (5), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), Immunoelectron Microscopy (IEM) (2)
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| Produktnummer |
ABIN314945 |
| Menge |
50 µg (0.95 mg/ml) (Varianten) |
| Preis |
410,00 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 7 bis 10 Werktagen |
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Weitere Bezeichnung
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Nestin
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Gen-ID
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10763, 18008, 25491
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Swiss-Prot
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P48681
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Immunogen
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Recombinant protein.
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Beschreibung
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Nestin, is a large intermediate filament protein (class Type VI) that was first identifiedwith a monoclonal antibody by Hockfield and McKay (1985). Nestin is expressedpredominantly in stem cells of the central nervous system in the neural tube. Nestin isexpressed during development and in myotendinous and neuromuscular junctions.Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be areasonable neuronal marker, however, recent studies have found nestin expression inother cell types such as endothelial cells. Nestin expression is seen in almost all GBMs(Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but notin any metastatic carcinomas. Upon terminal neural differentiation, nestin isdownregulated and replaced by neurofilaments. Alternate Names: anti-NES antibody, FLJ21841 antibody, NBLA00170 antibody.
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Spezifität
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Amino acids 1484-1500 of human Nestin protein. Species Reactivity: This antibody is reactive to Bovine, Dog, Hamster, Human, Macaque, Mouse, Rabbit,Rat.
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Applikationshinweise
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Suggested working dilutions: Western Blot 1:500-1:3000,ELISA 1:20000-1:80000,Immunohistochemistry-Paraffin 5 ug/ml
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Konzentration
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0.95 mg/ml
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Buffer
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PBS. Preservative: 0.01% Sodium Azide.
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Lagerung
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Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.
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Forschungsgebiet
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Angiogenese, Abstammungsmarker
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "Nestin (NES)", Typ "Antikörper" finden
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Wirte
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Kaninchen (80), Maus (30), Huhn (3), Ziege (1)
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Reaktivitäten
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Human (86), Maus (36), Ratte (Rattus) (32), Rind (Kuh) (6), Kaninchen (6), Hund (5), Pferd (4), Schwein (4), Huhn (1), Schimpanse (1), Hamster (1), Affe (1), Schaf (1)
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Applikationen
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Western Blot (WB) (70), Immunfluoreszenz (IF) (60), Durchflusszytometrie (FACS) (50), Immunhistochemie (Paraffinschnitte) (IHC (p)) (35), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (24), Immunpräzipitation (IP) (13), ELISA (12), Immunhistochemie (IHC) (11), Enzyme Immunoassay (EIA) (9), Immunzytochemie (ICC) (5), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), Immunoelectron Microscopy (IEM) (2)
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Konjugate
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Biotin (4), HRP (4), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2)
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Epitope
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C-Term (4), AA 1484-1500 (1), AA 544-776 (1)
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