Heat Shock Protein 70 (HSP70) (HSPA4) Antikörper

Antigen: Heat Shock Protein 70 (HSP70) (HSPA4)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Enzyme Immunoassay (EIA), Western Blot (WB)

Produktnummer: ABIN314649

Produktbeschreibung

Weitere Bezeichnung: Heat shock protein 70 / HSP70

Gen-ID: 3303, 24472

Beschreibung: The HSP 70 family is composed of four highly conserved proteins: HSP 70, HSC 70,GRP 75 and GRP 78. These proteins serve a variety of roles: they act as molecularchaperones facilitating the assembly of multi-protein complexes, participate in thetranslocation of polypeptides across cell membranes and to the nucleus and aid in theproper folding of nascent polypeptide chains. All members of the family, except HSP 70,are constitutively expressed in primate cells. HSP 70 expression is strongly induced inresponse to heat stress. HSP 70 and HSC 70 play key roles in the cytosolicendoplasmic reticulum and mitochondrial import machinery and are found in both thecytosol and nucleus of mammalian cells. Both HSP 70 and HSC 70 are involved in thechaperoning of nascent polypeptide chains and in protecting cells against theaccumulation of improperly folded proteins. GRP 78 is localized in the endoplasmicreticulum, where it receives imported secretory proteins and is involved in the foldingand translocation of nascent peptide chains. It has been postulated that members of theHSP 70 family act as force-generating motors, relying on the hydrolysis of ATP for theiractivity.

Spezifität: HSP 70 (F245) antibody detects endogenous levels of HSP 70 protein. Species Reactivity: This antibody is reactive against Human, Mouse, Rat.

Anwendungen

Applikationshinweise: Suggested working dilutions: ELISA 1:60000-1:80000,Western Blot 1:500-1:1000,immunohistochemistry 1:50-1:200

Konzentration: 1 mg/ml

Buffer: Phosphate buffered saline, pH 7.2. Preservative: 15 mM sodium azide.

Lagerung: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar