Acrosomal Vesicle Protein 1 (ACRV1) Antikörper

Antigen: Acrosomal Vesicle Protein 1 (ACRV1)

Synonyme: SP-10, SPACA2, D11S4365, Sp10, ACRV1, Msa63

Klonalität: Monoklonal (Hs-14)

Wirt: Maus

Reaktivität: Human, Maus

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN314188

Produktbeschreibung

Weitere Bezeichnung: ACRV1

Gen-ID: 56, 11451

Immunogen: Tissue / cell preparation (Human). Freshly ejaculated human sperms were washed inPBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acidextract was dialyzed against 0.2% acetic acid and subsequently used for immunization.

Isotyp: IgM  (Passende Sekundärantikörper)

Klon: Hs-14

Beschreibung: This protein is a testis-specific, differentiation antigen, acrosomal vesicle protein 1, thatarises within the acrosomal vesicle during spermatogenesis, and is associated with theacrosomal membranes and matrix of mature sperm. The gene encoding this proteinconsists of 4 exons and its alternative splicing generates 11 distinct transcripts, whichencode protein isoforms ranging from 81 to 265 amino acids. The longest transcript isthe most abundant, comprising 53-72% of the total acrosomal vesicle protein 1messages, the second largest transcript comprises 15-32%, the third and the fourthlargest transcripts account for 3.4-8.3% and 8.7-12.5%, respectively, and the remaining7 transcripts combined account for < 1% of the total acrosomal vesicle protein 1message. It is suggested that phenomena of cryptic splicing and exon skipping occurwithin this gene. The acrosomal vesicle protein 1 may be involved in sperm-zonabinding or penetration, and it is a potential contraceptive vaccine immunogen forhumans. Alternate Names: anti-acrosomal vesicle protein 1 antibody, anti-Msa63 antibody.

Spezifität: The antibody reacts with human intra-acrosomal protein (220 kDa). Species Reactivity: Cross-reacts with Human and Mouse. Not yet tested in other species.

Anwendungen

Applikationshinweise: Suggested working dilutions: flow cytometry / FACS analysis Neat, do not dilute

Buffer: TBS pH 7.4, with BSA. Preservative: Sodium Azide.

Lagerung: Store at 4C. Do not freeze. Store in the dark, do not expose to light long term.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Greenhalgh, Griffith, Wald: "The Use of Immunofluorescence in Microdissection Testicular Sperm Extraction." in: Journal of andrology, 2009 (PubMed).