Phospho-TSC2 (Thr1462) Antikörper

Antigen: Phospho-TSC2 (Thr1462)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Immunfluoreszenz (IF)

Produktnummer: ABIN314093

Produktbeschreibung

Immunogen: Modified peptide

Format: Purified

Klon: Poly6358

Beschreibung: Tuberous sclerosis complex (TSC) is an autosomal dominant geneticdisorder that is caused by mutation in either the TSC1 (hamartin) or TSC2(tuberin) gene. TSC1 and TSC2 proteins form a physical and functionalheterodimer in vivo that inhibits ribosomal protein S6 kinase 1 (S6K1) andactivates eukaryotic initiation factor 4E-binding protein 1 (4E-BP1) byinhibition of the mammalian target of rapamycin (mTOR). TSC2 belongs tosmall GTPase protein of the Ras superfamily, contain a Rap-GAP domain.Predicted molecular weight 201 kD. TSC2 is directly phosphorylated andinhibited by Akt that is involved in stimulating cell growth and is activated bygrowth stimulating signals, such as insulin. Thr1462 of TSC2 is Akt-regulatedphosphorylation site. The Poly6358 antibody recognizes humanphospho-TSC2 (Thr1462) and has been shown to be useful forimmunoflorence staining. This antibody does not detect TSC2phosphorylated at other sites.

Anwendungen

Applikationshinweise: Each lot of this antibody is quality control tested by immunofluorescencestaining. For immunofluorescence microscopy: Use a dilution range of1:100~1:400. For IHC, use a 1:50 dilution of antibody for staining. Antigenretrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 Msodium citrate buffer is recommended. It is recommended that the reagent betitrated.

Reinigung: This antibody was purified by affinity chromatography.

Buffer: This antibody is provided in phosphate-buffered solution, pH 7.2, containing0.09% sodium azide and 50% glycerol.

Lagerung: Upon receipt, store frozen at -20 degrees C.

Beschränkungen: Nur für Forschungszwecke einsetzbar