C-Fos Antikörper

Antigen: C-Fos

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Ratte (Rattus), Maus

Applikation: Western Blot (WB)

Produktnummer: ABIN313929

Produktbeschreibung

Immunogen: DMDLSGSFYAADWEC (aa. 291-304 of human c-Fos)Reactivity: HumanCross-

Format: Purified

Klon: Poly6414

Beschreibung: The c-fos protein, also known as FBJ murine viral (v-fos) oncogene homolog , is a 62 kD cellular oncogene. The c-fosprotein contains a bZIP region with a DNA-binding domain and a leucine zipper domain. Together with c-jun, c-junproteins form the heterodimeric AP-1 complex that binds to the TRE elements of cellular and viral genes includingcollagenase, interleukin 2, stromelysin, SV40 and polyoma among others. c-fos is expressed in a variety of normaladult tissues and has been implicated as a critical transcription factor for skeletal formation and maintenance. Thistranscription factor is modified by phosphorylation by a number of upstream kinases and has been shown to interactwith a variety of other proteins involved in transcriptional regulation. c-fos has been implicated in the development offibrous bone dysplasia and osteosarcoma. The c-fos protein has been shown to interact with a variety of otherproteins including c-jun, TFIID, NCOA2, NFAT1, CHOP, ELK1, SRC1, and ETS1. The Poly6244 antibody recognizeshuman, rat, and mouse c-fos and has been shown to be useful for Western blotting, immunoprecipitation, andimmunohistochemistry.

Anwendungen

Applikationshinweise: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s):Use 10 ul per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimalperformance for each application.

Reinigung: The antibody was purified by antigen-affinity chromatography.

Buffer: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.

Lagerung: Upon receipt, store at -20 degrees C.

Beschränkungen: Nur für Forschungszwecke einsetzbar