IL-5 Antikörper

Antigen: IL-5

Klonalität: Monoklonal (JES1-5A10)

Wirt: Ratte

Reaktivität: Human, Rhesusaffen

Applikation: ELISA

Produktnummer: ABIN313717

Produktbeschreibung

Immunogen: COS-expressed, recombinant human IL-5

Format: Purified

Isotyp: IgG2a, kappa chain  (Passende Sekundärantikörper)

Klon: JES1-5A10

Beschreibung: IL-5 is a homodimeric, disulphide-linked protein produced by T-cells. Monomeric human IL-5 is a 126 amino acidprotein with a reported molecular weight of 26 kD for the homodimeric protein. Mouse and human IL-5 areapproximately 70% identical. IL-5 has been shown to promote the growth of immature hematopoietic BFU-Eprogenitors, stimulate the activation and differentiation of eosinophils, and promote the generation of cytotoxiclymphocytes. The JES1-39D10 antibody can neutralize the bioactivity of natural or recombinant IL-5.

Anwendungen

Applikationshinweise: For use as an ELISA detection antibody, a concentration range of 0.25-1.0 ug/ml is recommended. To obtain a linearstandard curve, serial dilutions of IL-5 recombinant protein ranging from 500 to 4 pg/ml are recommended for eachELISA plate. It is recommended that the reagent be titrated for optimal performance for each application. ELISA1-3 or ELISPOT4-6 Detection: The biotinylated JES1-5A10 antibody is useful as a detection antibody for asandwich ELISA or ELISPOT assay, when used in conjunction with purified JES1-39D10 (Cat. No. 500902/500907)or TRFK5 (Cat. No. 504302/504308) antibody as the capture antibody. Flow Cytometry7: The fluorochrome-labelled JES1-5A10 antibody is useful for intracellular immunofluorescentstaining and flow cytometric analysis to identify IL-5 -producing cells within mixed cell populations. Neutralization1: The LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4 degrees C.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Mahanty, Abrams, King et al.: "Parallel regulation of IL-4 and IL-5 in human helminth infections." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 11, pp. 3567-71, 1992 (PubMed).

Abrams, Roncarolo, Yssel et al.: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, pp. Mai 24, 1992 (PubMed).

Butterfield, Leiferman, Abrams et al.: "Elevated serum levels of interleukin-5 in patients with the syndrome of episodic angioedema and eosinophilia." in: Blood, Vol. 79, Issue 3, pp. 688-92, 1992 (PubMed).

Limaye, Abrams, Silver et al.: "Interleukin-5 and the posttreatment eosinophilia in patients with onchocerciasis." in: The Journal of clinical investigation, Vol. 88, Issue 4, pp. 1418-21, 1991 (PubMed).

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996 (PubMed).