IL-10 Antikörper

Antigen: IL-10

Klonalität: Monoklonal (JES3-12G8)

Wirt: Ratte

Reaktivität: Human

Applikation: ELISA, Immunhistochemie (IHC)

Produktnummer: ABIN313698

Produktbeschreibung

Immunogen: COS-expressed recombinant human IL-10

Format: Purified

Isotyp: IgG2a, kappa chain  (Passende Sekundärantikörper)

Klon: JES3-12G8

Beschreibung: IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokineproduction by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. Thebiological activities of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayedtype hypersensitivity response, and stimulation of the Th2 cell response, which results in elevated antibodyproduction. The JES3-12G8 antibody reacts with human and viral interleukin-10 (IL-10). The JES3-12G8 antibody canneutralize the bioactivity of natural or recombinant IL-10.

Anwendungen

Applikationshinweise: For ELISA detection applications, a concentration range of 0.25-1.0 ug/ml is recommended. It is recommended thatthe reagent be titrated for optimal performance for each application. ELISA or ELISPOT1-4: JES3-12G8 antibody is useful as a detection antibody in a sandwich ELISA or ELISPOTassay, when used in conjunction with the purified JES3-3-9D7 antibody (Cat. No. 501402/501407) or purifiedJES3-19F1 antibody (Cat. No. 506802/506810) as capture antibody and recombinant human IL-10 (Cat. No. 561201)as the standard. Flow Cytometry: The fluorochrome-labeled JES3-12G8 antibody is useful for intracellular immunofluorescent stainingand flow cytometric analysis to identify IL-10 -producing cells within mixed cell populations. Neutralization1,2: The LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4 degrees C.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Yssel, De Waal Malefyt, Roncarolo et al.: "IL-10 is produced by subsets of human CD4+ T cell clones and peripheral blood T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 7, pp. 2378-84, 1992 (PubMed).

Abrams, Roncarolo, Yssel et al.: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, pp. Mai 24, 1992 (PubMed).

Gotlieb, Abrams, Watson et al.: "Presence of interleukin 10 (IL-10) in the ascites of patients with ovarian and other intra-abdominal cancers." in: Cytokine, Vol. 4, Issue 5, pp. 385-90, 1992 (PubMed).

Andersson, Abrams, Bjoerk et al.: "Concomitant in vivo production of 19 different cytokines in human tonsils." in: Immunology, Vol. 83, Issue 1, pp. 16-24, 1995 (PubMed).

Burdin, Pueronne, Banchereau et al.: "Epstein-Barr virus transformation induces B lymphocytes to produce human interleukin 10." in: The Journal of experimental medicine, Vol. 177, Issue 2, pp. 295-304, 1993 (PubMed).