CD20 (cytoplasmic) Antikörper

Antigen: CD20

Epitope: cytoplasmic

Klonalität: Monoklonal (1412)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN313570

Produktbeschreibung

Weitere Bezeichnung: CD20 (cytoplasmic)

Format: Purified

Isotyp: IgG2a, kappa chain  (Passende Sekundärantikörper)

Klon: 1412

Beschreibung: CD20 is a 33-37 kD four transmembrane spanning protein also known as B1 and Bp35. CD20 is expressed onpre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cellsubset. CD20 is heavily phosphorylated in activated B cells and malignant B cells. Homo-oligomeric complexes ofCD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule isinvolved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn), and exists in a complexwith MHC class I and II, CD53, CD81, and CD82. The mAb 1412 recognizes the cytoplasmic domain of the CD20molecule.

Anwendungen

Applikationshinweise: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is approx. 1.0 ug per 106 cells in 100 ul volume. It isrecommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4 degrees C.

Beschränkungen: Nur für Forschungszwecke einsetzbar