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CD80 (CD80) Antikörper (PerCP/Cy5.5)

Antigen

CD80 (CD80)
Synonyme LAB7, CD28LG, CD28LG1
Klonalität Monoklonal (16-10A1)
Wirt
Alternativen

Hamster
Reaktivität
Konjugat
Alternativen PerCP,Cy5.5
Applikation
Alternativen Durchflusszytometrie (FACS)
Produktnummer ABIN313479
Menge 25 ug  (Varianten)
Preis 122,86 €   Zzgl. Versandkosten €20,00 und MWSt
Lieferung nach
Verfügbarkeit Lieferung in 5 bis 7 Werktagen

Produktbeschreibung
Anwendungen Alternativen Varianten zurück nach oben

Weitere Bezeichnung CD80
Immunogen CHO cell line transfected with mouse B7 (CD80)
Isotyp IgG
Klon 16-10A1
Beschreibung CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily, also known as B7-1, B7 and Ly-53.CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed onactivated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (suchas dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation,proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells. The 16-10A1 antibody hasbeen shown to block the co-stimulation of T cells by activated B cells in vitro and to block the binding of CTLA-4 toCD80.

Anwendungen
Alternativen Varianten zurück nach oben

Applikationshinweise Each lot of this antibody is quality control tested by immunofluorescentstaining with flow cytometric analysis. For immunofluorescent staining, thesuggested use of this reagent is approx. 0.25 ug per 106 cells in 100 ul volume. It isrecommended that the reagent be titrated for optimal performance for eachapplication. * PerCP/Cy5.5 has a maximum absorption of 482 nm and 564 nm and amaximum emission of 690 nm. Additional reported applications (for the relevant formats) include: immunoprecipitation2, in vitro and in vivo blocking2-4 of CTLA-4 Ig to CD80, and immunohistochemical staining1,4 of acetone-fixed frozen sections. The LEAF
Reinigung The antibody was purified by affinity chromatography, and conjugated withPerCP/Cy5.5 under optimal conditions. The solution is free of unconjugatedPerCP/Cy5.5 and unconjugated antibody.
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Lagerung The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.
Beschränkungen Nur für Forschungszwecke einsetzbar

Alternativen
Anwendungen Varianten zurück nach oben

Alternativen zu Antigen "CD80 (CD80)", Typ "Antikörper" finden
Wirte Maus (174), Ratte (69), Hamster (62), Kaninchen (55), Ziege (2)
Reaktivitäten Maus (163), Human (161), Ratte (Rattus) (46), Huhn (6), Hund (4), Kaninchen (3), Pavian (2), Rind (Kuh) (2), Pferd (2), Schwein (2)
Applikationen Durchflusszytometrie (FACS) (304), Immunpräzipitation (IP) (71), Western Blot (WB) (62), Immunfluoreszenz (IF) (54), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (39), Funktionale Studien (Func) (25), ELISA (24), Immunhistochemie (IHC) (16), Enzyme Immunoassay (EIA) (11), Immunhistochemie (Paraffinschnitte) (IHC (p)) (11), Blockierende Antikörper (Inhibition) (7), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), Immunzytochemie (ICC) (3), Dot Blot (Dot) (2), ELISA (Detektionsantikörper) (2), Immunoelectron Microscopy (IEM) (2), Neutralisierung (Neut) (1)
Konjugate PE (47), Biotin (45), FITC (44), APC (14), PE,Cy5 (11), Alexa Fluor 488 (7), Alexa Fluor 647 (7), Cy5,PE (6), RPE (3), SPRD (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), Alexa Fluor 700 (1), Pacific Blue (1), PerCP (1), PerCP,Cy5.5 (1), RPE,Cy5 (1)
Epitope AA 45-63 (1), extra-cellular domain (1)