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Lymphocyte Antigen 6 Complex, Locus G (Ly6g) Antikörper (PerCP)

Antigen

Lymphocyte Antigen 6 Complex, Locus G (Ly6g)

Synonyme Gr1, Gr-1, Ly-6G
Klonalität Monoklonal (RB6-8C5)
Wirt
Alternativen

Ratte

Reaktivität
Konjugat
Alternativen PerCP
Applikation
Alternativen Durchflusszytometrie (FACS)
Produktnummer ABIN313281
Menge 25 ug  (Varianten)
Preis 111,43 €   Zzgl. Versandkosten €20,00 und MWSt
Lieferung nach
Verfügbarkeit Lieferung in 5 bis 7 Werktagen

Produktbeschreibung

Weitere Bezeichnung Ly-6G (Gr-1)
Isotyp IgG2b, kappa chain
Klon RB6-8C5
Beschreibung Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is aglycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. In bone marrow, theexpression levels of Gr-1 directly correlate with granulocyte differentiation and maturation, Gr-1 is also transientlyexpressed on bone marrow cells in the monocyte lineage. Gr-1+ cells have recently been implicated in thedevelopment of antitumor responses. The RB6-8C5 antibody has been used to identify peripheral blood neutrophilsand deplete granulocytes in vivo.

Anwendungen

Applikationshinweise Each lot of this antibody is quality control tested by immunofluorescentstaining with flow cytometric analysis. For immunofluorescent staining, thesuggested use of this reagent is approx. 0.25 ug per 106 cells in 100 ul volume. It isrecommended that the reagent be titrated for optimal performance for eachapplication.* PerCP has a maximum absorption of 482 nm and 564 nm and a maximumemission of 675 nm. Additional reported applications (for the relevant formats) include: in vitro complement-mediated cytotoxicity2, in vivodepletion3-5,9, immunoprecipitation1, immunohistochemical staining6,9 of zinc-fixed paraffin-embedded sections andacetone-fixed frozen sections, and Western blotting7. Special Note: This antibody is not recommended forformalin-fixed paraffin-embedded sections. The LEAF
Reinigung The antibody was purified by affinity chromatography, and conjugated withPerCP under optimal conditions. The solution is free of unconjugated PerCPand unconjugated antibody.
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Lagerung The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.
Beschränkungen Nur für Forschungszwecke einsetzbar

Alternativen

Alternativen zu Antigen "Lymphocyte Antigen 6 Complex, Locus G (Ly6g)", Typ "Antikörper" finden
Wirte Ratte (49), Kaninchen (1)
Reaktivitäten Maus (50)
Applikationen Durchflusszytometrie (FACS) (49), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (8), Immunhistochemie (Paraffinschnitte) (IHC (p)) (8), Western Blot (WB) (7), Immunpräzipitation (IP) (6), Immunfluoreszenz (IF) (4), Abreicherung (Depletion) (2), Immunzytochemie (ICC) (1)
Konjugate PE (6), Biotin (5), FITC (5), Alexa Fluor 647 (4), APC (3), APC,Cy7 (3), PE,Cy7 (3), Alexa Fluor 488 (2), Alexa Fluor 700 (2), PE,Cy5 (2), PerCP,Cy5.5 (2), Cy5 (1), PerCP (1), SPRD (1)