CD90.2 Antikörper (PerCP)

Antigen: CD90.2

Klonalität: Monoklonal (30-H12)

Wirt: Ratte

Reaktivität: Maus

Konjugat: PerCP

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN313268

Produktbeschreibung

Immunogen: Mouse thymus or spleen

Isotyp: IgG2b, kappa chain

Klon: 30-H12

Beschreibung: CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy-1.2. It is expressed on hematopoieticstem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J,C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoproteininvolved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction ofhematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45. The 30-H12 antibody hasbeen reported to induce Ca2+ flux in thymocytes and, in combination with antibody against the CD3/TCR complex,promote thymocyte apoptosis and inhibit CD3-mediated proliferative responses of mature T lymphocytes.

Anwendungen

Applikationshinweise: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is approx. 0.25 ug per 106 cells in 100 ul volume. It isrecommended that the reagent be titrated for optimal performance for each application.* PerCP has a maximum absorption of 482 nm and 564 nm and a maximum emission of 675 nm. Additional reported applications (for the relevant formats) include: in vivo and in vitro depletion1,2,7, costimulation ofCD3/TCR-mediated signal transduction3,4, and immunohistochemical staining5 of acetone-fixed frozen sections. The30-H12 antibody does not react with Thy-1.1 alloantigen of the AKR/J and PL strains. To reduce non-specific bindingto cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No.101301/101302), is recommended prior to immunofluorescent staining. The LEAF

Reinigung: The antibody was purified by affinity chromatography, and conjugated withPerCP/Cy5.5 under optimal conditions. The solution is free of unconjugatedPerCP/Cy5.5 and unconjugated antibody.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.

Beschränkungen: Nur für Forschungszwecke einsetzbar