Siglec H Antikörper (Alexa Fluor 647)

Antigen: Siglec H

Klonalität: Monoklonal (551)

Wirt: Ratte

Reaktivität: Maus

Konjugat: Alexa Fluor 647

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN311994

Produktbeschreibung

Immunogen: Siglec-H-Fc recombinant fusion protein

Isotyp: IgG1

Klon: 551

Beschreibung: The Siglec-H is a member of CD33-related Siglec family which mostly expressed in the innate immune system. Siglec-H is reported to be specifically expressed on plasmacytoid dendritic cells. Siglec-H depends on adaptorprotein DAP 12 (DNAX-activating protein of 12Kd) for surface expression and signal transduction. Cross-linking withthis antibody 440c can selectively inhibit interferon-alpha production by IPCs following TLR9 ligation.

Anwendungen

Applikationshinweise: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is approx. 0.25 ug per 106 cells in 100 ul volume. It isrecommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by affinity chromatography, and conjugated withPE under optimal conditions. The solution is free of unconjugated PE andunconjugated antibody.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Blasius, Cella, Maldonado et al.: "Siglec-H is an IPC-specific receptor that modulates type I IFN secretion through DAP12." in: Blood, Vol. 107, Issue 6, pp. 2474-6, 2006 (PubMed).

Zhang, Raper, Sugita et al.: "Characterization of Siglec-H as a novel endocytic receptor expressed on murine plasmacytoid dendritic cell precursors." in: Blood, Vol. 107, Issue 9, pp. 3600-8, 2006 (PubMed).

Blasius, Barchet, Cella et al.: "Development and function of murine B220+CD11c+NK1.1+ cells identify them as a subset of NK cells." in: The Journal of experimental medicine, Vol. 204, Issue 11, pp. 2561-8, 2007 (PubMed).