CD194 (CCR4) Antikörper (Alexa Fluor 647)

Antigen: CD194 (CCR4)

Klonalität: Monoklonal (TG6-CCR4)

Wirt: Maus

Reaktivität: Human

Konjugat: Alexa Fluor 647

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN311834

Produktbeschreibung

Immunogen: Human CCR4 transfectans

Isotyp: IgG2b, kappa chain

Klon: TG6-CCR4

Beschreibung: CCR4 is a CC chemokine receptor, and it was clone from a human basophylic cell line KU-812 in 1995. (1). CCR4binds CCL17 and CCL22 and is expressed on stimulated and unstimulated T, and B cells, basophils, monocytes, andNK cells. Human Th2 cells are characterized by the expression of CCR4 and CCR8 (2), and these receptors areregulated differently during Th2 development (3). Human peripheral blood Tregs can be divided into two distinctpopulations based on the expression of CCR4. Freshly isolated Tregs express CCR4 and presumably representmemory-type Tregs, and CCR4(-) Tregs require anti-CD3 Ab-mediated activation to acquire a regulatory activity.Depletion of CCR4(+) T cells leads to Th1-type polarization of CD4(+) T cells and augmentation of CD8(+) T cellresponses to tumor Ags (4). CCR4 and its ligands are important for the recruitment of memory T cells into the skin invarious cutaneous immune diseases (5).

Anwendungen

Applikationshinweise: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 ul per million cells or 5 ul per 100 ul of wholeblood. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor

Reinigung: The antibody was purified by affinity chromatography, and conjugated withAlexa Fluor 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor 647.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and0.2% (w/v) BSA (origin USA).

Lagerung: The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do not freeze.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-CD194 (CCR4) antibody (Alexa Fluor 647)