Panendothelial Cell Antigen Antikörper (Alexa Fluor 488)

Antigen: Panendothelial Cell Antigen

Klonalität: Monoklonal (MECA-32)

Wirt: Ratte

Reaktivität: Maus

Konjugat: Alexa Fluor 488

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN311807

Produktbeschreibung

Immunogen: Mouse lymph node stromal cells

Isotyp: IgG2a, kappa chain

Klon: MECA-32

Beschreibung: MECA-32 is a 50-55 kD homodimer glycoprotein primarily expressed on most endothelial cells in the embryonic andthe adult mouse tissues, except in the brain and in skeletal and cardiac muscle, where it has more restricteddistribution. In skeletal and cardiac muscle, the MECA-32 antigen is confined in small arterioles and venules. But insome conditions, such as inflammation or cardiac transplantation, MECA-32 expression can be induced on somenegative-cardiac endothelial cells. In the brain, its expression occurs only in the circumventricular organs and theneurohemal tissues. During embryonic development, MECA-32 antigen is expressed on the brain vasculature up today 16 of gestation, and disappears at day 17 and after.

Anwendungen

Applikationshinweise: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is approx. 1.0 ug per 106 cells in 100 ul volume. It isrecommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor

Reinigung: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor 488 under optimalconditions. The solution is free of unconjugated Alexa Fluor 488.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4 degrees C and protected from prolonged exposure to light. Do notfreeze.

Beschränkungen: Nur für Forschungszwecke einsetzbar