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Antikörper
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anti-Nanog Homeobox Pseudogene 8 (NANOGP8) Antikörper
Nanog Homeobox Pseudogene 8 (NANOGP8) Antikörper
| Antigen |
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| Synonyme |
ENK, ecat4, 2410002E02Rik, PN8, NANOG, NANOGP1, MGC119250, nanog |
| Klonalität |
Monoklonal (5A10) |
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Wirt
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Reaktivität
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Human (126), Maus (37), Ratte (Rattus) (26), Rind (Kuh) (5), Hund (5), Schwein (4), Huhn (3), Kaninchen (2), Zebrafisch (Brachydanio rerio) (2), Ziege (1), Pferd (1), Schaf (1)
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Applikation
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Western Blot (WB) (97), Immunfluoreszenz (IF) (53), Durchflusszytometrie (FACS) (42), ELISA (36), Enzyme Immunoassay (EIA) (29), Immunhistochemie (Paraffinschnitte) (IHC (p)) (18), Immunhistochemie (IHC) (8), Immunpräzipitation (IP) (8), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), Dot Blot (Dot) (2), Immunzytochemie (ICC) (2), Immunoelectron Microscopy (IEM) (2), Blockierende Antikörper (Inhibition) (1), ELISA (Detektionsantikörper) (1), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (1)
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| Produktnummer |
ABIN306525 |
| Menge |
50ul (1 mg/ml) |
| Preis |
230,77 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 5 bis 7 Werktagen |
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Weitere Bezeichnung
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Nanog
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Immunogen
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Recombinant human Nanog (1-154aa) purified from E. coli. Anti-human Nanog mAb, clone 5A10, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Nanog protein.
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Isotyp
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IgG2a kappa (Passende Sekundärantikörper)
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Klon
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5A10
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Beschreibung
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Nanog is a multidomain homeobox transcription factor that functions to maintain the undifferentiated state of pluripotent stem cells. Nanog expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. When Nanog expression is downregulated, cell differentiation can proceed. Proteins that regulate Nanog expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53.
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Applikationshinweise
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The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for is 1:500 ~ 1:2000.Recommended starting dilution is 1:500.
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Konzentration
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1 mg/ml
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Reinigung
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The antibody was purified from mouse ascitic fluids by protein-G affinity chromatography. Can be stored at 4C for up to one month, but store at -20C for long term storage. Avoid repeated freezing and thawing cycles.
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Buffer
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Liquid in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "Nanog Homeobox Pseudogene 8 (NANOGP8)", Typ "Antikörper" finden
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Wirte
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Kaninchen (99), Maus (26), Ziege (10), Ratte (3)
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Reaktivitäten
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Human (126), Maus (37), Ratte (Rattus) (26), Rind (Kuh) (5), Hund (5), Schwein (4), Huhn (3), Kaninchen (2), Zebrafisch (Brachydanio rerio) (2), Ziege (1), Pferd (1), Schaf (1)
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Applikationen
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Western Blot (WB) (97), Immunfluoreszenz (IF) (53), Durchflusszytometrie (FACS) (42), ELISA (36), Enzyme Immunoassay (EIA) (29), Immunhistochemie (Paraffinschnitte) (IHC (p)) (18), Immunhistochemie (IHC) (8), Immunpräzipitation (IP) (8), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), Dot Blot (Dot) (2), Immunzytochemie (ICC) (2), Immunoelectron Microscopy (IEM) (2), Blockierende Antikörper (Inhibition) (1), ELISA (Detektionsantikörper) (1), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (1)
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Konjugate
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Biotin (7), Alexa Fluor 488 (3), Alexa Fluor 647 (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2)
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Epitope
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C-Term (4), Center (3), N-Term (2), S285 (2), S71 (1)
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Alternativen 
