PCNA Antikörper

Antigen: PCNA

Klonalität: Monoklonal (K1A3)

Wirt: Maus

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN306516

Produktbeschreibung

Immunogen: Recombinant human PCNA (1-261aa) purified from E. coli. Anti-human PCNA mAb, clone k1A3, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PCNA protein.

Isotyp: IgG3 kappa  (Passende Sekundärantikörper)

Klon: K1A3

Beschreibung: PCNA (Proliferating Cell Nuclear Antigen) is found in the nucleus and is a cofactor of DNA polymerase delta. This protein is associated with DNA synthesis and repair. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. It appears during late G1- phase, S-phase of mitosis and persists until the end of the M-phase because of its long biological half-life. PCNA may be induced by UV irradiation, growth factors and eventually by neighboring tumors. Concentration: 1 mg/ml

Anwendungen

Applikationshinweise: The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for is 1:1,000 ~ 1:3,000.Recommended starting dilution is 1:2,000.

Reinigung: The antibody was purified from mouse ascitic fluids by protein-G affinity chromatography. Can be stored at 4C for up to one month, but store at -20C for long term storage. Avoid repeated freezing and thawing cycles.

Buffer: Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide

Forschungsgebiet: Proliferationsmarker

Beschränkungen: Nur für Forschungszwecke einsetzbar