SDHB Antikörper (N-Term)

Produktnummer ABIN3044278

Produktdetails anti-SDHB Antikörper

Target: SDHB (Succinate Dehydrogenase Complex, Subunit B, Iron Sulfur (Ip) (SDHB))

Bindungsspezifität: N-Term

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SDHB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Succinate dehydrogenase[ubiquinone] iron-sulfur subunit, mitochondrial(SDHB) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: FAIYRWDPDK AGDKPHMQ

Kreuzreaktivität (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for Succinate dehydrogenase[ubiquinone] iron-sulfur subunit, mitochondrial(SDHB) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: succinate dehydrogenase complex, subunit B, iron sulfur(Ip)
Protein Name: Succinate dehydrogenase[ubiquinone] iron-sulfur subunit, mitochondrial

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SDHB(42-59 FAIYRWDPDKAGDKPHMQ), different from the related rat sequence by one amino acid and mouse sequence by two amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu SDHB

Target: SDHB (Succinate Dehydrogenase Complex, Subunit B, Iron Sulfur (Ip) (SDHB))

Andere Bezeichnung: SDHB (SDHB Produkte)

Synonyme: succinate dehydrogenase 2-3 antikoerper, CG3283 antikoerper, Dmel\\CG3283 antikoerper, Ip antikoerper, SDH antikoerper, SDH-IP antikoerper, SDH-Ip antikoerper, SDHb antikoerper, sdhB antikoerper, CWS2 antikoerper, IP antikoerper, PGL4 antikoerper, SDH1 antikoerper, SDH2 antikoerper, SDHIP antikoerper, 0710008N11Rik antikoerper, fj46d06 antikoerper, wu:fj46d06 antikoerper, succinate dehydrogenase complex iron sulfur subunit B antikoerper, succinate dehydrogenase [ubiquinone] iron-sulfur subunit 3 antikoerper, Succinate dehydrogenase, subunit B (iron-sulfur) antikoerper, succinate dehydrogenase complex subunit B, iron sulfur (Ip) antikoerper, succinate dehydrogenase complex, subunit B, iron sulfur (Ip) antikoerper, succinate dehydrogenase complex subunit B, iron sulfur (Ip) S homeolog antikoerper, SDHB antikoerper, SDH2-3 antikoerper, SdhB antikoerper, sdhb antikoerper, Sdhb antikoerper, sdhb.S antikoerper

Hintergrund: SDHB(Succinate Dehydrogenase Complex, Subunit B, iron sulfur protein), also known as iron-sulfur subunit of complex II(Ip) or SDH2, HOMOLOG OF, is a protein that in humans is encoded by the SDHB gene. SDHB is one of four protein subunits forming succinate dehydrogenase, the other three being SDHA, SDHC and SDHD. The SDHB subunit is connected to the SDHA subunit on the hydrophilic, catalytic end of the SDH complex. The SDHB gene is mapped on 1p36.13. The entire SDHB transcript is encoded by 8 exons within approximately 40 kb by Au et al(1995). Pollard et al.(2005) stated that the nuclear-encoded Krebs cycle enzymes fumarate hydratase and succinate dehydrogenases like SDHB act as tumor suppressors, and germline mutations in these genes predispose individuals to leiomyomas and renal cancer and to paragangliomas, respectively. In affected members of families with paragangliomas-4, mutations were identified in the SDHB gene.

Synonyms: CWS2 antibody|DHSB_HUMAN antibody|FLJ92337 antibody|IP antibody|Iron sulfur subunit antibody|Iron sulfur subunit of complex II antibody|Iron-sulfur subunit of complex II antibody|mitochondrial antibody|OTTHUMP00000044624 antibody|PGL 4 antibody|PGL4 antibody|SDH 1 antibody|SDH antibody|SDH1 antibody|SDH2 antibody|SDHB antibody|SDHIP antibody|Succinate dehydrogenase[ubiquinone] iron sulfur protein mitochondrial antibody|Succinate dehydrogenase[ubiquinone] iron-sulfur subunit antibody|succinate dehydrogenase[ubiquinone] iron-sulfur subunit, mitochondrial antibody|Succinate Dehydrogenase 1 Iron Sulfur Subunit antibody|Succinate dehydrogenase complex subunit B iron sulfur antibody|Succinate Dehydrogenase Complex Subunit B Iron Sulfur Protein antibody|succinate dehydrogenase complex, subunit B, iron sulfur(Ip) antibody|Succinate dehydrogenase iron sulfur protein antibody

UniProt: P21912