TJP2 Antikörper (C-Term)

Produktnummer ABIN3044036

Produktdetails anti-TJP2 Antikörper

Target: TJP2 (Tight Junction Protein 2 (Zona Occludens 2) (TJP2))

Bindungsspezifität: AA 1149-1167, C-Term

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TJP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Tight junction protein ZO-2(TJP2) detection. Tested with WB, IHC-P in Mouse,Rat.

Sequenz: AHSKRGYYSQ PSRYRDTEL

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Tight junction protein ZO-2(TJP2) detection. Tested with WB, IHC-P in Mouse,Rat.
Gene Name: tight junction protein 2
Protein Name: Tight junction protein ZO-2

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse TJP2(1149-1167aa AHSKRGYYSQPSRYRDTEL), identical to the related rat sequence.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Rat, The detection limit for TJP2 is approximately 2.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu TJP2

Target: TJP2 (Tight Junction Protein 2 (Zona Occludens 2) (TJP2))

Andere Bezeichnung: TJP2 (TJP2 Produkte)

Synonyme: C9DUPq21.11 antikoerper, DFNA51 antikoerper, DUP9q21.11 antikoerper, X104 antikoerper, ZO2 antikoerper, ZO-2 antikoerper, zo2 antikoerper, tjp2 antikoerper, x104 antikoerper, zo-2 antikoerper, wu:fb62b09 antikoerper, zgc:92094 antikoerper, tight junction protein 2 antikoerper, tight junction protein 2 L homeolog antikoerper, tight junction protein 2b (zona occludens 2) antikoerper, TJP2 antikoerper, Tjp2 antikoerper, tjp2.L antikoerper, tjp2b antikoerper

Hintergrund: TJP2(Tight Junction Protein 2), also known as Zona Occludens 2 or ZO2, is a protein that in humans is encoded by the TJP2 gene. Tight junction proteins(TJPs) belong to a family of membrane-associated guanylate kinase(MAGUK) homologs that are involved in the organization of epithelial and endothelial intercellular junctions. Duclos et al.(1994) mapped the TJP2 gene telomeric to the Friedreich ataxia critical region on chromosome 9q13-q21. TJP2 lies about 70 kb centromeric to the X123 gene and is transcribed in the centromere-to-telomere direction. Using in vitro assays and immunoprecipitation studies, Itoh et al.(1999) showed that the mouse Tjp1, Tjp2, and Tjp3 PDZ1 domains interacted with the C-terminal cytoplasmic domains of Cldn1 through Cldn8. In the mouse inner ear, Walsh et al.(2010) found that Tjp2 expression decreased rapidly between E16.5 and age 1 week to a level in adult mice that was approximately 50 % of the level at birth(P0).

Synonyms: Friedreich ataxia region gene X104(tight junction protein ZO-2) antibody|MGC26306 antibody|Tight junction protein 2 antibody|Tight junction protein ZO 2 antibody|Tight junction protein ZO-2 antibody|TJP2 antibody|X104 antibody|ZO 2 antibody|ZO-2 antibody|ZO2 antibody|ZO2_HUMAN antibody|Zona occludens protein 2 antibody|Zonula occludens protein 2 antibody

UniProt: Q9Z0U1