APP Antikörper (C-Term)

Produktnummer ABIN3043787

Produktdetails anti-APP Antikörper

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Bindungsspezifität: AA 672-713, C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser APP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Amyloid beta A4 protein(APP) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: DAEFRHDSGY EVHHQKLVFF AEDVGSNKGA IIGLMVGGVV IA

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Amyloid beta A4 protein(APP) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: amyloid beta (A4) precursor protein
Protein Name: Amyloid beta A4 protein

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human APP(672-713aa DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA), different from the related mouse and rat sequences by three amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for APP is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Referenzen für anti-APP Antikörper (ABIN3043787)

Sun, Zhou, Yi, Jiang, Yuan: "Lead-induced morphological changes and amyloid precursor protein accumulation in adult rat hippocampus." in: Biotechnic & histochemistry : official publication of the Biological Stain Commission, Vol. 89, Issue 7, pp. 513-7, (2014) (PubMed).

Qin, Yao, Huang: "Effects of compound danshen tablets on spatial cognition and expression of brain beta-amyloid precursor protein in a rat model of Alzheimer's disease." in: Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan / sponsored by All-China Association of Traditional Chinese Medicine, Academy of Traditional Chinese Medicine, Vol. 32, Issue 1, pp. 63-6, (2012) (PubMed).

Li, Li, Huang, Kan, Wang, Wu, Yin, Yao: "Dexamethasone and A???-?? accelerate learning and memory impairments due to elevate amyloid precursor protein expression and neuronal apoptosis in 12-month male rats." in: Behavioural brain research, Vol. 227, Issue 1, pp. 142-9, (2011) (PubMed).

Nie, Luo, Huang, Gong, Wu, Shi: "Icariin inhibits beta-amyloid peptide segment 25-35 induced expression of beta-secretase in rat hippocampus." in: European journal of pharmacology, Vol. 626, Issue 2-3, pp. 213-8, (2009) (PubMed).

Details zu APP

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Andere Bezeichnung: APP (APP Produkte)

Synonyme: AAA antikoerper, ABETA antikoerper, ABPP antikoerper, AD1 antikoerper, APPI antikoerper, CTFgamma antikoerper, CVAP antikoerper, PN-II antikoerper, PN2 antikoerper, aaa antikoerper, abeta antikoerper, abpp antikoerper, ad1 antikoerper, appi antikoerper, ctfgamma antikoerper, cvap antikoerper, pn2 antikoerper, APP antikoerper, APP-like antikoerper, APPL antikoerper, Abeta antikoerper, BcDNA:GH04413 antikoerper, CG7727 antikoerper, Dmel\\CG7727 antikoerper, EG:65F1.5 antikoerper, appl antikoerper, Abpp antikoerper, Adap antikoerper, Ag antikoerper, Cvap antikoerper, E030013M08Rik antikoerper, betaApp antikoerper, app antikoerper, wu:fj34d10 antikoerper, wu:fk65e12 antikoerper, zgc:85740 antikoerper, amyloid beta precursor protein antikoerper, amyloid beta (A4) precursor protein antikoerper, beta amyloid protein precursor-like antikoerper, amyloid beta (A4) precursor protein a antikoerper, amyloid beta precursor protein L homeolog antikoerper, APP antikoerper, app antikoerper, Appl antikoerper, App antikoerper, appa antikoerper, app.L antikoerper

Hintergrund: Beta Amyloid, also called Abeta or Abeta, denotes peptides of 36-43 amino acidsthat are crucially involved in Alzheimer's disease as the main component of the amyloid plaques found in the brains of Alzheimer patients. It is mapped to 19q13.12. Several potential activities have been discovered for beta Amyloid, including activation of kinase enzymes, functioning as atranscription factor, and anti-microbial activity (potentially associated with beta Amyloid's pro-inflammatoryactivity). Moreover, monomeric beta Amyloid is indicated to protect neurons by quenching metal-inducible oxygen radical generation and thereby inhibiting neurotoxicity.

Synonyms: A4 antibody|A4 antibody|A4_HUMAN antibody|AAA antibody|AAA antibody|ABETA antibody|ABETA antibody|ABPP antibody|ABPP antibody|AD1 antibody|AICD-50 antibody|AICD-57 antibody|AICD-59 antibody|AID(50) antibody|AID(57) antibody|AID(59) antibody|Alzheimer disease amyloid protein antibody|Alzheimers Disease Amyloid Protein antibody|Amyloid B antibody|amyloid beta (A4) precursor protein antibody|amyloid beta A4 protein antibody|Amyloid Beta A4 Protein Precursor antibody|Amyloid Beta antibody|Amyloid intracellular domain 50 antibody|Amyloid intracellular domain 57 antibody|Amyloid intracellular domain 59 antibody|Amyloid of Aging and Alzheimer Disease antibody|APP antibody|APPI antibody|APPI antibody|B Amyloid antibody|Beta APP antibody|beta-amyloid peptide antibody|Beta-APP40 antibody|Beta-APP42 antibody|C31 antibody|Cerebral Vascular Amyloid Peptide antibody|CTFgamma antibody|CVAP antibody|CVAP antibody|Gamma-CTF(50)antibody|Gamma-CTF(57) antibody|Gamma-CTF(59) antibody|peptidase nexin-II antibody|PN II antibody|PN-II antibody|PN2 antibody|PreA4 antibody|PreA4 antibody|Protease nexin II antibody|Protease nexin-II antibody|S-APP-alpha antibody|S-APP-beta antibody

Gen-ID: 351

UniProt: P05067

Pathways: Caspase Kaskade in der Apoptose, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour