CDK4 Antikörper (AA 201-303)

Produktnummer ABIN3043745

Produktdetails anti-CDK4 Antikörper

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Bindungsspezifität: AA 201-303

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDK4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Cyclin-dependent kinase 4(CDK4) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Cyclin-dependent kinase 4(CDK4) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: cyclin-dependent kinase 4
Protein Name: Cyclin-dependent kinase 4

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Cdk4 recombinant protein (Position: G201-E303). Human Cdk4 shares 94.2% amino acid (aa) sequence identity with both mouse and rat Cdk4.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Referenzen für anti-CDK4 Antikörper (ABIN3043745)

Ren, Huang, Xu, Yang, Yang, Hu: "Isoflavone lupiwighteone induces cytotoxic, apoptotic, and antiangiogenic activities in DU-145 prostate cancer cells." in: Anti-cancer drugs, Vol. 26, Issue 6, pp. 599-611, (2015) (PubMed).

Zhou, Lu, Liu, Guo, Liu, Zhou, Yang, Mi, Xu: "Platycodin D induces tumor growth arrest by activating FOXO3a expression in prostate cancer in vitro and in vivo." in: Current cancer drug targets, Vol. 14, Issue 9, pp. 860-71, (2015) (PubMed).

Zhang, Zhao, Huang, Meng, Bian, Li: "Melittin restores PTEN expression by down-regulating HDAC2 in human hepatocelluar carcinoma HepG2 cells." in: PLoS ONE, Vol. 9, Issue 5, pp. e95520, (2014) (PubMed).

Wang, Huang, Han, Xue, Li, Liu, Shan, Wang, Li, Li: "The effects of artemisinin on the proliferation and apoptosis of vascular smooth muscle cells of rats." in: Cell biochemistry and function, Vol. 32, Issue 2, pp. 201-8, (2014) (PubMed).

Li, Xu, Chu, Gao, Wang, Nie, Yang, Lv: "Molecular mechanism of inhibitory effects of CD59 gene on atherosclerosis in ApoE (-/-) mice." in: Immunology letters, Vol. 156, Issue 1-2, pp. 68-81, (2013) (PubMed).

Cai, Xu, Zhang: "Bone marrow stromal cells induce cell cycle arrest in reactive astrocytes in vitro." in: Neuroscience letters, Vol. 522, Issue 1, pp. 62-6, (2012) (PubMed).

Li, Xu, Li, Zou, Saimaiti, Simayi, Wang, Zhang, Yuan, Zhang: "Stat6 activity-related Th2 cytokine profile and tumor growth advantage of human colorectal cancer cells in vitro and in vivo." in: Cellular signalling, Vol. 24, Issue 3, pp. 718-25, (2012) (PubMed).

Zhang, Ge, Sun, Cao, Wu, Guo, Wang: "Effect of bone morphogenetic protein-2 on proliferation and apoptosis of gastric cancer cells." in: International journal of medical sciences, Vol. 9, Issue 2, pp. 184-92, (2012) (PubMed).

Liu, Dai, Lu, Wei, Ha, Rong, Mu, You, Li, Guo: "LYG-202 inhibits the proliferation of human colorectal carcinoma HCT-116 cells through induction of G1/S cell cycle arrest and apoptosis via p53 and p21(WAF1/Cip1) expression." in: Biochemistry and cell biology = Biochimie et biologie cellulaire, Vol. 89, Issue 3, pp. 287-98, (2011) (PubMed).

Han, Liu: "EGR-1 decoy ODNs inhibit vascular smooth muscle cell proliferation and neointimal hyperplasia of balloon-injured arteries in rat." in: Life sciences, Vol. 86, Issue 7-8, pp. 234-43, (2010) (PubMed).

Details zu CDK4

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Andere Bezeichnung: CDK4 (CDK4 Produkte)

Synonyme: zgc:153726 antikoerper, CMM3 antikoerper, PSK-J3 antikoerper, Crk3 antikoerper, 8-6 antikoerper, CDK4 antikoerper, CDK4/6 antikoerper, CG5072 antikoerper, Cdk4/6 antikoerper, DmCdk4 antikoerper, Dmel\\CG5072 antikoerper, Pk53C antikoerper, Pk?7 antikoerper, cdk antikoerper, cdk4 antikoerper, cdk4/6 antikoerper, dCdk4 antikoerper, l(2)05428 antikoerper, l(2)0671 antikoerper, l(2)k06503 antikoerper, l(2)s4639 antikoerper, l(2)sh0671 antikoerper, xcdk4 antikoerper, cyclin-dependent kinase 4 antikoerper, cyclin dependent kinase 4 antikoerper, Cyclin-dependent kinase 4 antikoerper, cyclin-dependent kinase 4 L homeolog antikoerper, cdk4 antikoerper, CDK4 antikoerper, Cdk4 antikoerper, cdk4.L antikoerper

Hintergrund: Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. Human cell division is regulated primarily at the G1-to-S or the G2-to-M boundaries within the cell cycle. The complexes formed by CDK4 and the D-type cyclins are involved in the control of cell proliferation during the G1 phase. CDK4 is inhibited by p16, also known as cyclin-dependent kinase inhibitor-2. CDK4 is mapped to 12q14. And CDK4 expression and activity are required for cytokine responsiveness in T cells.

Synonyms: Cdk 4 antibody|cdk4 antibody|CDK4 protein antibody|CDK4_HUMAN antibody|Cell division kinase 4 antibody|Cell division protein kinase 4 antibody|CMM 3 antibody|CMM3 antibody|Crk3 antibody|Cyclin dependent kinase 4 antibody|Cyclin-dependent kinase 4 antibody|Melanoma cutaneous malignant 3 antibody|MGC14458 antibody|p34 cdk4 antibody|PSK J3 antibody|PSK-J3 antibody

Gen-ID: 1019

UniProt: P11802

Pathways: Zellzyklus, Mitotic G1-G1/S Phases, Regulation of Cell Size